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by: Sydney Notetaker

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Sydney Notetaker

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This 1 page Class Notes was uploaded by Sydney Notetaker on Wednesday July 20, 2016. The Class Notes belongs to at Bradley University taught by in Summer 2016. Since its upload, it has received 20 views.


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Date Created: 07/20/16
Biol 210 Test 4 Study Guide DNA stuff, Microbial Genetics, and Gene Transfer  1) Know the basic terminology. Understand the BASIC structure of DNA. Understand the  “transfer of genetic information”: replication, transcription, translation and what’s going on. Protein synthesis occurs at _______.  Gene expression is manifested in the production of  ______ encoded by _____. 2) What is the difference between a genotype and a genome? 3) Protein synthesis ­ understand it a) order of events of protein synthesis: roles of DNA, mRNA, rRNA, and tRNA b) relationship of information encoded on nucleotide bases, mRNA codons, tRNA  anticodons, and amino acids c) gene regulation (operons) : constitutive and regulated genes; induction and repression  d) Mutations: i) What are they? What are the types and the possible effects? Mutations alter what  cellular product(s)? ii) Causes of mutation: spontaneous mutations and mutation via various mutagens.  iii) All mutations are "bad": yes/ no. 4) Regulation of gene expression­  understand it a) Induction  b) Repression c) What's being regulated: ___________. 5) What IS genetic recombination? What’s a clone? What’s a vector as it relates to genetic  recombination? 6) Understand a) Vertical and horizontal (lateral) gene transfer  i) Vertical= normal multiplication ii) Horizontal= transfer gen material 1 bact to another b) Methods of (horizontal) gene transfer: what are they  i) Transformation: naked DNA ii) Conjugation: via plasmids & sex pili. F factors and R factors are: what? iii) Transduction: via bacteriophage c) Plasmids and transposons d) Genes and evolution e) Why do we care about this stuff in bacteria?  Understand how microbes become antibiotic resistant and the consequences. Anti­microbial Drugs: see chapter notes. 1


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