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Cellular Respiration Tester

by: Nicole Notetaker

Cellular Respiration Tester CHM-112-01

Marketplace > Camden County College > Chemistry > CHM-112-01 > Cellular Respiration Tester
Nicole Notetaker


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About this Document

Chemistry II
Dr. Roberto Feudale
Class Notes
Science, Biology
25 ?




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This 5 page Class Notes was uploaded by Nicole Notetaker on Wednesday August 17, 2016. The Class Notes belongs to CHM-112-01 at Camden County College taught by Dr. Roberto Feudale in Fall 2016. Since its upload, it has received 7 views. For similar materials see Chemistry II in Chemistry at Camden County College.


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Date Created: 08/17/16
Cellular Respiration Introduction: Our bodies need fuel to function. The fuel we need to function comes from the food we eat in forms of proteins, carbohydrates, and fats. In order for our body to be able to use that energy that comes from the food we eat it must be converted into adenosine triphosphate also known as ATP. To make the ATP energy our body needs glucose molecules are split in a process called cellular respiration. Equation for Cellular Respiration C H O + 6O  6CO + 6H O + energy 6 12 6 2 2 2 Cellular Respiration occurs in the mitochondria of eukaryotic cells and in the cell membrane of prokaryotic cells. The ATP that is made by Cellular Respiration provides the energy needed for synthetic reactions, active transport and all cell processes. Equation for the breakdown of glucose Glucose + Oxygen  Carbon Dioxide + Water + Energy (ATP) Cellular Respiration will only occur if oxygen is present in the cell. The complete oxidation of glucose by Cellular Respiration yields a total of 36 to 38 ATP molecules. Respiration applies to two distinct but interrelated processes: the active acquisition of oxygen by an organism and the release of energy by the breakdown of organic compounds by metabolic chemical oxidation within cells. Various factors such as temperature change affect respiration. Oxygen consumption during respiration can be measured with a respirometer as a change in gas volume. The physical laws related to gases are important when using a respirometer. The general gas law is PV=nRT where P: pressure V: volume n: number of molecules R: gas content T: temperature The purpose of this experiment is to investigate the effect of germination or non-germination and temperature on cellular respiration. Hypothesis: I believe the rate of cellular respiration will be greater in the germinating peas and that the decrease in temperature will cause a decrease in the rate of cellular respiration. Materials:  20 Germinating peas  20 Dry peas  A bag of Glass beads  6 mL of Potassium hydroxide 15%  2 Waterbaths  6 Respirometers (glass vials, graduated pipets, one-hole stoppers, washers)  6 Absorbent cotton balls  6 Nonabsorbent cotton balls  1 Graduated cylinder  2 Thermometers  1 Pipet  Ice  Petroleum jelly Procedures: 1.Set up an ice waterbath in a large tray and keep the tray fill with ice at all times. Add a thermometer. Chill the water t less than 10C and maintain this temperature throughout the experiment. Also, set up a room temperature waterbath in another large tray. 2.Obtain six vials with steel washers on the bottoms. 3.Fill a 100 mL graduated cylinder with 50 mL of water. Add 10 germinating peas and take a reading of the displaced water. This is the volume of the germinating peas. Record the volume. Decant the water, remove the peas and place them on a paper towel; pat the peas dry and set aside. 4.Refill the graduated cylinder with 50 mL of water. Add 10 dry non-germinating peas. Add glass beads until the water level is the same as that of the germinating peas. Remove the peas and beads and place them on a paper towel; pat the peas and beads dry and set aside. 5.Refill the graduated cylinder with 50 mL of water. Add glass beads until the water level is the same as that of the germinating peas. Remove the beads and place them on a paper towel; pat the beads dry and set aside. 6.Repeat steps 3 through 5 with more germinating peas, non- germinating peas and beads, and beads. Set this aside for vials 4-6. 7.Place an absorbent cotton ball in each of the six vials and push each down to the bottom using a pipet or pencil tip. 8.Without getting any liquid on the sides of the respirometers, use a pipet to add 1 mL of 15% potassium hydroxide (KOH) to the cotton. Add a piece of non-absorbent rayon that is slightly smaller than that of the cotton ball and place it on top of the KOH-soaked cotton. 9. Using the first set of germinating peas, non-germinating peas and glass beads, and glass beads, place them in vials 1-3, respectively. Repeat this procedure using your second set of germinating peas, non-germinating peas and glass beads, and glass beads for vials- . 10. Insert the non-tapered end of a graduated pipet into the wide end of a stopper so that the tapered end of the pipet is furthest from the stopper and so that the pipet extends just beyond the bottom of the stopper. 11. Firmly insert the stopper into the vial. The seal that has been created between the stopper and the vial should be sufficient enough to prevent the pipet from easily moving up and down in the stopper. Place a washer over the pipet tip and guide it down the pipet until it rests on the stopper. Repeat this entire step for the remaining five vials. 12. Place vials 1-3 in the room temperature waterbath with the pipet tips resting on the edge of the tray. Place vials 4-6 in the chilled waterbath in the same manner. Allow all the respirometers to equilibrate for 10 minutes. 13. Read all of the respirometers to the nearest 0.01 mL and take the temperature of each waterbath. Record the initial readings and the temperature of each waterbath. 14. Take additional readings every five minutes for 30 minutes abd record the readings and temperature. 15. When all of the readings have been taken, calculate the difference and corrected difference for each result and record each value. Variable & Controls: Results & Analysis: Results Summary: Sources of Error: Conclusion: Improvements: Questions:


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