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Microbiology 201, Wednesday 14, 2016 Notes

by: Laura Notetaker

Microbiology 201, Wednesday 14, 2016 Notes Microbiology 201

Marketplace > Pennsylvania State University > Microbiology > Microbiology 201 > Microbiology 201 Wednesday 14 2016 Notes
Laura Notetaker
Penn State

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About this Document

Class discussion focused on : Enriched Medium & Microbial Growth.
Dr. Ola Sodeinde
Class Notes
Biology, Microbiology
25 ?




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This 2 page Class Notes was uploaded by Laura Notetaker on Saturday September 17, 2016. The Class Notes belongs to Microbiology 201 at Pennsylvania State University taught by Dr. Ola Sodeinde in Fall 2016. Since its upload, it has received 9 views. For similar materials see Microbiology in Microbiology at Pennsylvania State University.

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Date Created: 09/17/16
September14,2016Notes EnrichedMedium 1. Defined/Synthetic a. Knownchemical composition 2. Complex a. Unknownchemicalcomposition,but "rich" (ex: McConkeys) 3. Enriched a. Containsreagent forgrowthoffastidious(picky)microbes (Ex: Blood agar, Charcoalagar) 4. Selective a. Permits growthof only certain microbes within amixed population i. McConkeyscontainsbile salts ii. Gram positives are sensitive iii. Gram negatives areresistant 5. Differential a. Distinguishes between subsetsof microbes i. Ex: McConkeyscontainsdyeto distinguish between lactosefermenting and non- fermenting microbes // AlsoBlood agar MICROBIALGROWTH 1. Growth a. Increasein acell's components i. Usually followedby binary fissionin bacteria ii. Most eukaryoticcells dividefollowing mitosis b. In bacteria, growth in thelaboratory is measured as the increase in cell populationovertime i. Graph knownas Growth Curve 2. Growth Curve a. Occurs in a "closed" systemorbatch culture; no addition ofnutrients orremovalof waste products i. Thus,overtime (nutrients)decrease; (wasteproducts)increase. ii. Plotted as log10IABLEcell# vs.Time b. 4 Phases i. Lag ii. Exponential (Log) iii. Stationary iv. Death 1. Lag Phase a. No immediateincrease in cell # b. Synthesisof new cellular componentseg. ATP, ribosomes,DNA,etc. c. Variable in duration d. Occurs when cells are transferred i. From old cultureto fresh medium ii. From oneto adifferent typeofmedium 2. Exponential Phase a. Maximal growthrate b. Constantgrowthrate i. Cell # doublesat regular timeintervals c. Phaseofgrowthwhen cell population is most chemically and physically uniform. 3. Stationery Phase a. # viableorganisms remain constant i. Death rate balanced by growthrate b. Dueto: i. Depletion ofnutrients, and accumulation ofwasteproducts. 4. Death Phase a. Decrease in viablecount b. Severe depletion of nutrientsand accumulation of wasteproducts. c. Also logarithmicdecline in viable cell # 3. Indicesof Growth a. Doubling orGeneration Time i. Measured during exponentialphase ii. Time foratwo-foldincreasein cell # 1. Ex: E.coli doublingtime ~30 mins In 24hours~ 1 x10 cells (1gram) 28 In 48hours1 x 10 cells (7grams) b. Growth RateConstant i. # Generations/ Unit time(Ex: generations perhour) 4. MeasurementofMicrobial Growth a. Direct Counting i. Count# cells in achamberof knownvolume(Ex: Petroff-HauserChamber) b. Advantage:Quick &simple c. Disadvantage: NOTof viable cell count unless a vital stain is used. 5. ElectronicCounting a. CoulterCounter b. Also NOTviable in cell count.


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