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Crossing Over in Genetics

by: Smccarty

Crossing Over in Genetics ABT 360

Marketplace > University of Kentucky > GENE - Genetics > ABT 360 > Crossing Over in Genetics
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Linkage analyses in genetics, chromosome mapping, cytogenetic mapping, recombination and evolution
Dr. Bruce Webb
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This 3 page Class Notes was uploaded by Smccarty on Saturday September 24, 2016. The Class Notes belongs to ABT 360 at University of Kentucky taught by Dr. Bruce Webb in Fall 2016. Since its upload, it has received 4 views. For similar materials see Genetics in GENE - Genetics at University of Kentucky.

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Date Created: 09/24/16
th September 20  Notes: Linkage Analyses in Genetics Independent assortment­what allows you to start looking at the same chromosomes but  different genes Results of this violates Mendel's law of independent assortment (coupling of sweet peas and demonstrates LINKAGE) recombination happens, if independently assorted, and spread very far from each other on the chromosome  then it happens about 80% of the time. The if recombination happens between two alleles that you are tracking then you have the recombination event .  More parental types happen than expected  Recombination "hot spot" : recombination is likely to occur in the same spot most every  time.  Question in class: When is DNA is more than  likely   N  be subject to recombination? Centromere for example  Your frame of reference is what you  can see, by what genes you are  following.   Morgan's lab, beads on a string example: but how you demonstrate that changer is happening? Corn, chromosome 9. Anyone can visual track the chromosome of that physical abnormality. Looking for the movement in the copying process.  th September 20  Notes: Linkage Analyses in Genetics Problems with experiment : Pieces of chromosome could react differently, the  recombination will be frequently enough and that crossing over caused recombination.  Another factor : You have your genes too far apart from each other. The data doesn't indicate that they are related. Multiple crossing over: happens at meiosis I. Need to have markers, to bridge the gap or else.  The data is homogenized. One of the tricks used is inversions. The result is a chromosome with  two centromeres and one with none. Flipping the section, suppression or balancing  chromosomes. Two point crosses: is there a way to tell where they are on the chromosome. Estimating between  the gene: chromosome mapping.  50% is the highest recombination you can get, why? How many alleles are you studying? ­ Two.  To study gene order, you need more than two genes.  Chromosome Mapping : Linked genes can be mapped on a chromosome by studying how often their alleles recombine.  Recombination Frequency establishes genetic distance between alleles and is correlated  with physical distance   Calculation of map distances: parental type alleles will be the most predominant. Double  recombination are much less frequent than single recombinant. Progeny number lets you  figure the distance between the alleles. One can estimate the distance between genes: 2­Point Testcross; Measure parental vs non­parental phenotypes Determining gene order: based upon which one is in the middle Example of a Three­Point Test Cross Taken from the test a couple years ago. Lowest number is the double recombinant. Highest  would be the parental.  .                                                P is in the middle September 20  Notes: Linkage Analyses in Genetics Parentals will be 2 highest, second is the single recombinant, third is the double recombinant: then taking those alleles and make a map. Why didn't he do the x chromosome? Cryogenic mapping: deletion mapping, and duplication mapping Impact off recombination? Occurs in every animal and it's frequencies change constantly. High radiation does can amp up the gene recombination.  Heterochromatic knob First structure is abnormal, second is normal  Genetic markers (WX and C)  (Demonstrated that recombination of alleles involved  physical transfer of parts of the chromosome) Another part of the chromosome 


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