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Uconn MCB2210 Notes

by: linchen.deng Notetaker

Uconn MCB2210 Notes MCB 2210 001

linchen.deng Notetaker
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About this Document

This note covers the last lecture material that is going to be on midterm on Sep.30th.
Cell Biology
David Daggett (PI)
Class Notes




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This 1 page Class Notes was uploaded by linchen.deng Notetaker on Monday September 26, 2016. The Class Notes belongs to MCB 2210 001 at University of Connecticut taught by David Daggett (PI) in Fall 2016. Since its upload, it has received 9 views. For similar materials see Cell Biology in MCBA - Cell Biol & Anatomy at University of Connecticut.

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Date Created: 09/26/16
MCB2210 Week 5 Monday Enzyme-linked receptors involve receptor multimerization  Example: Receptor Tyrosine Kinases(RTK)  Ligand outside the cell makes single kinases to dimerize, they come together to form a new structure that is connected by cross-phosphorylation  Then active catalytic domain is exposed or associated enzyme is activated for transduction Two ways to activate phospholipase C and IP3/calcium signaling 1. GPCR(G-protein-coupled receptors) 2. Activated RTKs MAP kinase cascade stimulates the nucleus to cause cells to proliferate Small G protein  Example: G alpha proteins that bind to GDP and GTP in GPCR, Ras-activating protein.  Accessory proteins that control the activation status of the small G proteins  GEF- cause GDP to release and allow GTP to bind  GDI- prevent GDP from coming of  GAP-encourages GTPase to cleave GTP to turn of the activity  Signaling via phosphorylation and via small G protein both involve releasing and bind of the phosphate group. Signal can be activated by binding or repressed by cleavage of the phosphate group. Activated Ras proteins have downstream efects  It continues to activate MAP kinase kinase kinase, which activates MAP kinase kinase, which activates MAP kinase. These series of processes require phosphorylation.  Then diferent protein factors are activated. Some of them trigger change other proteins’ activity, and some of them act as a regulator, causing changes in gene expression.


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