New User Special Price Expires in

Let's log you in.

Sign in with Facebook


Don't have a StudySoup account? Create one here!


Create a StudySoup account

Be part of our community, it's free to join!

Sign up with Facebook


Create your account
By creating an account you agree to StudySoup's terms and conditions and privacy policy

Already have a StudySoup account? Login here

General Biochemistry, note set 5

by: Thomas Salazar

General Biochemistry, note set 5 4115

Thomas Salazar
Virginia Tech
GPA 3.32

Preview These Notes for FREE

Get a free preview of these Notes, just enter your email below.

Unlock Preview
Unlock Preview

Preview these materials now for free

Why put in your email? Get access to more of this material and other relevant free materials for your school

View Preview

About this Document

These notes include material for exam 2, enzyme kinetics, enzyme inhibition, Lineweaver-Burke plots, and enzyme mechanisms
General Biochemistry
Dr. Richard Helm
Class Notes
biochemistry, Enzymes, kinetics, Enzyme inhibition, competitive inhibitor, uncompetitive inhibitor, non-competitive-inhibitor, lineweaver-burke-plot
25 ?




Popular in General Biochemistry

Popular in Biochemistry

This 2 page Class Notes was uploaded by Thomas Salazar on Sunday October 9, 2016. The Class Notes belongs to 4115 at Virginia Polytechnic Institute and State University taught by Dr. Richard Helm in Fall 2016. Since its upload, it has received 18 views. For similar materials see General Biochemistry in Biochemistry at Virginia Polytechnic Institute and State University.


Reviews for General Biochemistry, note set 5


Report this Material


What is Karma?


Karma is the currency of StudySoup.

You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!

Date Created: 10/09/16
Note set #5 – week 7 General Biochemistry ENZYME KINETICS CONTINUED E + S ↔ES ↔ EP ↔ E + P  1 order rxns: ???????? o one substrate goes to one product: A ↔ P and ln = -Kt ????????  2 ndorder rxns: st 2 o 2 [1 order substrate] goes to two products: 2A ↔ P + Q r = K[A] or, o Two 2 ndorder substrates go to two products: A + B ↔ P + Q r = K[A][B] Where “r” is the reaction rate, and “K” is the reaction rate constant  Formulas for Enzyme Kinetics: ????????????????[????] o V =0 ????????+[????], where K m [S] when V = ½ V max  Low K m high affinity for ES complex, high K lmw affinity for ES o K cat= turnover rate constant ????????????????  Kcat= , where [Et is total enzyme concentration [????????] *organic systems do not operate atmax; but usually closer to maxin order to allow for easier adjustment of the enzymatic rate in either direction*  Lineweaver-Burke (LB) Equation: a plot of 1/V vs. 1/[S] 1 = ???????? ( ) + 1  y = mx + b ???? ???????????????? ???? ] ???????????????? o The y-intercept of this graph is 1/V max o The x-intercept is 1/K m ???????? o The term ???????????? is equal to the slope of the line  Enzyme Inhibition: o Irreversible: covalently bonded to enzyme until it is degraded, very difficult to dose properly in drug applications o Reversible:  Competitive: binds active site of E, inhibits the formation of ES complex  In an LB plot, the apparent V maxis unchanged, but the apparent K m does change  This causes the slope of the line to increase with [I], but the y-intercept remains unchanged [????]  The slope is modified by a value, α = (1+ ????????  Non-competitive (mixed): binds active site of E and/or binds ES complex  In LB plot, the apparent V max changes, and depending on the rxn, the apparent K mmy or may not change  This causes both the slope and the y-intercept to change with [I] Note set #5 – week 7 General Biochemistry [????]  The slope is modified by the value α = (1+ ), and the y-int. is modified ???? ???? by the value α’ = (1+ )????′ ????????  Uncompetitive: binds ES complex only  In LB plot, the apparent V maxis changed, and the apparent K alsm changes  This causes the slope to remain the same, but the y-int. increases with [I]  The y-int. is modified by the value α = (1+ )[????] ????????  Kinetics with Two Substrates: o Single Displacement: one substrate binds the E, followed by a second. The substrates are modified, then released.  Ordered sequential – one substrate must bind first for the rxn to occur, this substrate binds first, and is released last E + A  EA + B  EAB ↔ EPQ – P  EQ – Q  E , has rate constant K 1  Random sequential – either substrate can bind first and be released o Double displacement/ping-pong: a covalent intermediate from initial substrate remains and modifies the enzyme for following substrate  Substrate A binds enzyme E, a covalent intermediate from A binds E to form EA. EA forms enzyme-product complex FP. As P is released, F binds substrate B to form FB. FB forms enzyme-product complex EQ. As Q is released, the original enzyme E remains. E + A  EA ↔ FP – P  F + B  FB ↔ EQ – Q  E , has rate constants K 1 and 2  Transition State Theory: o Enzymes optimize for transition state o Must bind in proper orientation to the catalytic site o Molecular environment geometrically forces substrate to adopt transition state o All components, amino acids, cofactors, must be present for rxn to proceed Moonlighting – one protein fills multiple functions and roles in an organic system  Fructose-1,6-biphosphate aldolase o Central enzyme in glycolysis o Class I: mammals/plants – covalent catalysis o Class II: microbes


Buy Material

Are you sure you want to buy this material for

25 Karma

Buy Material

BOOM! Enjoy Your Free Notes!

We've added these Notes to your profile, click here to view them now.


You're already Subscribed!

Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'

Why people love StudySoup

Bentley McCaw University of Florida

"I was shooting for a perfect 4.0 GPA this semester. Having StudySoup as a study aid was critical to helping me achieve my goal...and I nailed it!"

Anthony Lee UC Santa Barbara

"I bought an awesome study guide, which helped me get an A in my Math 34B class this quarter!"

Jim McGreen Ohio University

"Knowing I can count on the Elite Notetaker in my class allows me to focus on what the professor is saying instead of just scribbling notes the whole time and falling behind."


"Their 'Elite Notetakers' are making over $1,200/month in sales by creating high quality content that helps their classmates in a time of need."

Become an Elite Notetaker and start selling your notes online!

Refund Policy


All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email


StudySoup has more than 1 million course-specific study resources to help students study smarter. If you’re having trouble finding what you’re looking for, our customer support team can help you find what you need! Feel free to contact them here:

Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to

Satisfaction Guarantee: If you’re not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.

Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.