OChem, Week 6
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This 14 page Class Notes was uploaded by Pratiti Ghosh-Dastidar on Sunday October 9, 2016. The Class Notes belongs to 203 at Miami University taught by in Fall 2016. Since its upload, it has received 6 views. For similar materials see Cell Biology in Biology at Miami University.
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Date Created: 10/09/16
Pg – 48 Movement of ions o Affected by the membrane potential separation of charges across the membrane (electron gradient) o Also affected by concentration gradient When membrane potential equalizes concentration gradient, it will cause no ne movement of ions even if there is a concentration gradient Membrane transport proteins o Helps more subs across membrane o Contains hydrophobic and hydrophilic sides o Made up of many . 2 types o Transporters o Channel Difference? o One needs sig conform change o the way it distinguishes substrate o Based on size and charge Can if fit through the fluid hole β charge Will have polar and/or ionized side chain inside channel o Substrate must be able to fit through binding pocket interaction req cause conform change of protein Pg – 50 Note: want to be lower b/cit can work faster with a lower [ ] to reach its full potential (Vmax) higher affinity binding/interaction b/w protein and subs is higher b/c protein A has a higher affinity for glucose (or whatever substrate) than B. Passive and Active Transport o Difference Passive Transport uses energy from the [ ] or electrochemical gradient. o Diffusional mechanics Ex) simple diffusion, channel, transporter o Active: requires input of additional energy not in gradient Usually moves substrate against energy gradient (low high [ ]) Sources of add energy o Light (sunlight), ATP hydrolysis to ADP, energy stored in the gradient of 2ndsubstrate One substrate moves light driven pumps down its [ ] grad. To ATP Pumps energize movement of other subs. ndcoupled pumps (2 ary active transporters) o Common? All moving subs. Against electrochem grad. All have conform change via binding rxn Requires add. energy source Pg – 51 Transporters Dif mem dif transporters giving them dif function. plasma membrane has different function than organelles Ex) interior of lysosome has + H pumps allowing to change pH Types: 1) Uniport: Moves 1 solute across membrane . a. If passive, then depends on [ ] gradient Ex) sugar transporter b. Has binding site specifically to type of substrate binding rxn interacts via noncovalent bonds. c. Undergoes conform change so it can move across membrane. d. Substrate never interact with hydrophobic portion of bilayer. e. If active, causes other source for energy i. Coupled pumps can be uniport 1. They move 2 or more across bilayer either 2) Symporters: a. Moving 2 or more subs in same direction 3) Antiports: a. Moving 2 or more subs in opposite direction. i. Does not have to be coupled Pg -52 Glucose Transport: A case study Gut lumen into fluid surrounding gut Blood fluid (muscle cell) in muscle cell Goes through multiple cells in order to get to muscle cell 1. Gut Lumen Active transport 2. Get Passive 3. Passive 4. Blood [low Glu] Passive 5. Passive 6. Muscle Cell Maintain using the glucose Pg – 53 1 2 Active transport Coupled pump + Uses Na gradient to move glucose against gradient. Ex) symport Also transport upto 250 H2O molecules. (Glucose transporters) GLUTS Faciliated diffusial uniporter for glucose Switch back and forth Na+ pump pumps out Na+ which delta which maintains low Na+ [ ] so the SCLT can work o Active pump coupled w/ K+ ions Also passive, mediated by GLUTs Mucle cell actively uses glucose. So lower [ ] in cell allowing GLUTs to mediate transport
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