Microscopes and Cell Lab
Microscopes and Cell Lab 18519
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This 4 page Class Notes was uploaded by Shanell Coleman on Monday October 10, 2016. The Class Notes belongs to 18519 at Augusta State University taught by Thomas Buxton in Fall 2016. Since its upload, it has received 6 views. For similar materials see Biology 1101 in Science at Augusta State University.
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Date Created: 10/10/16
Lab #3 Microscopes and Cell Lab 1. The light source is found in the base of the microscope (which bears the weight of the microscope). It is activated by turning on the light switch just in front of the light source. 2. The iris diaphragm is located just above the light source on the bottom side of the stage. Using the lever attached to it you can increase or decrease the amount of light reaching the specimen. 3. Between the stage and the iris diaphragm is the condenser. The condenser further aids in the focusing of the light onto the specimen. It can be moved up and down by the black knob called the condenser knob that is located on the right side of the stage. 4. Above the condenser lies the stage. It is mounted at a right angle to the arm and positioned just below the nosepiece. The stage is where you will place your specimen. It is through the movement of the stage up and down that you will bring your specimen into focus. 5. Resting on top of the stage is the mechanical stage. This contains a spring clip that will hold the slide in place. To the right of the mechanical stage are the two control knobs that allow you to move the slide left and right and backwards and forwards. This will enable you to look at all areas of the specimen. 6. At the back of the stage is the arm of the microscope that supports the head of the microscope. It is connected to the base and if a good place for you to grab hold of the microscope when you need to carry it or lift it out of its storage cabinet. 7. Attached to the arm are the coarse and fine adjustment knobs. These knobs move the stage up and down for the purpose of focusing the specimen. The course adjustment knob moves the stage a large visible distance with a single turn and as such should be used only with 4X and 10X objectives. It should NEVER be used with the 40X and 100X objectives. You run the risk of damaging these objectives and breaking the slide if you do not heed this warning. The fine adjustment knob is used to move the stage up or down only very slightly. Since these scopes are parfocal (all the objective lens focus the image in the same plane) once you have focused your specimen at the 4X or 10X then when you progress to the next objective you will only need to use the fine focus to make the minor adjustment needed for the specimen. Red 4X Yellow 10X Blue (Highest) 400X Bacteria (Cheek Cell) - Bacterial cells typically come in one of three shapes: spheres (coccus), rods (bacillus), or spirals (spirillum). - Methylene Blue used for contrast Parts of a cell (some) - Cell membrane: a phospholipid bilayer with the embedded protein that forms the boundary of all cells - Cytoplasm: gelatinous, aqueous interior - Nucleus: Organelle containing genetic material - Organelle: membrane – bound compartments of eukaryotes Eukaryotic Cells: Animal Cell (Cheek Cell) Eukaryotic Cells (Onion Cell): Plant 1. How are eukaryote cells different from prokaryote cells? Prokaryotes have no nucleus, no membrane bound organelles. Eukaryotes have nucleus, membrane bound organelles. 2. How does the size of a prokaryote compare to a eukaryote? Prokaryote – simple and small. Eukaryote – Big and Complex 3. How are anima cells and plant cells different? Plants cells have a cell wall and animal cells have a cell membrane. 4. How are animal cells and plant cells similar? Both are eukaryotic.