Chapter 7: DNA, structure, replication, profiling, & PCR
Chapter 7: DNA, structure, replication, profiling, & PCR BIO 101
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This 2 page Class Notes was uploaded by Hailey N Detrick on Monday October 17, 2016. The Class Notes belongs to BIO 101 at Elon University taught by in Fall 2016. Since its upload, it has received 6 views. For similar materials see Topics in General Biology in Biology at Elon University.
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Date Created: 10/17/16
Chapter 7: DNA, Structure, Replication, PCR, & Profiling 1. DNA Structure: a. 1950s: isolated things out of blood, found 2 molecules: i. Adeline: thymine ii. Guanine: cytosine b. 1952: x-ray picture of a “molecule” of DNA i. still not sure what it is c. 1953: double helix structure d. DNA: i. D: eoxyribose ii. N: ucleic iii. A:cid 1. Nucleic + Acid = base pairs a. A = T (compliment base pairs) b. C = G (compliment base pairs) e. Helicase: i. Unzips the helix, unwinding the DNA f. Polymerase: uses the unzipped DNA to make two helix g. Semi-Conservative: i. Parent + new = ½ parent + ½ new = DNA strand h. Conservative i. Parent + new = 1 new = DNA strand i. 1993: PCR: many copies of small pieces of DNA i. P:olymerase ii. C:hain iii. R:eaction iv. Steps: 1. Denaturization – using heat at 95° C a. Double helix à single helix 2. Annealing – using primer at 50° C 3. Extension: 72° C, using polymerase 2. DNA Profiling: DNA finger printing a. Genotype: pairs of chromosomes (23) –or- (46 singular chromosomes) b. Steps of DNA Profiling: i. DNA: blood, saliva, hair, skin cells, sperm, nails 1. Cheek swab (most common) ii. PCR: many copies 1. Primer on the STRs iii. Run Gel: 1. 1 STR = 1/5 chance of matching 2. 2 STR = 1/25 chance of matching 3. 3 STR = 1/3125 chance of matching 4. 4 STR = 1/350,000,000,000 chance of matching a. The higher the # of STR the more likely the DNA results match a singular person 3. Dead Sea Scrolls a. Found in caves around the dead sea b. 1947 found c. 2,000 years old d. bacteria will decompose them because they are made on goat skin e. used the same goats for all the scrolls i. suspect all of the scrolls came from 1 herd of sheep 1. #profiling
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