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by: Ezequiel Orn


Marketplace > University of Texas at Austin > Biology > BIO 325L > LAB EXPERIENCE IN GENETICS
Ezequiel Orn
GPA 3.89

John Batterton

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John Batterton
Class Notes
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This 5 page Class Notes was uploaded by Ezequiel Orn on Sunday September 6, 2015. The Class Notes belongs to BIO 325L at University of Texas at Austin taught by John Batterton in Fall. Since its upload, it has received 186 views. For similar materials see /class/181719/bio-325l-university-of-texas-at-austin in Biology at University of Texas at Austin.




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Date Created: 09/06/15
FLY LAB 2 PATTERNS OF INHERITANCE PART 5 PRELAB QUESTION SET 1 Was the eye color phenotype Visible in any of the F1 progeny What does this tell you about the patterns of inheritance of these traits Justify your answer N o This should tell you that the trait is recessive If you see any mutation phenotype in the F1 progeny this means that the trait is dominant 2 You made crosses using F1 siblings from each unknown You know the white eye phenotype in one of the vials is caused by a recessive mutation in the sex linked white w gene Using proper Drosophila notation write the a genotypes of the parental generation Female Male w Y b genotype of the F1 siblings used for this cross Female w Male Y c expected genotypes in the F2 progeny Just showing chromosome 1 Female w and Male w Yor Y d phenotypic ratios expected in the F2 progeny Females 100 wild type males 11 white eyexwild type e null hypothesis which describes the expected results for this experiment Some variation on the following The white w gene is sexlinked recessive so will produce all wild type females and half wild type half white eyed males in the F2 generation The prediction is that if the above is true then we should see certain numbers of ies based on the total counted 3 White eyes can also be due to the combination of two mutations which eliminate both pigment types normally found in the Drosophila eye Assume for now that the two mutations are autosomal recessive and linked In the space below use proper Drosophila notation and write the a genotypes of the parental generation Male 39 cncn bwbw 39 Female 39 39 39 b genotypes of the F1 siblings used for this cross Just showing chromosome 2 on bw c genotypes expected in the F2 progeny This question is really asking for 1 00 linkage so we should see or cn bw or cncn bwbw d phenotypic ratios expected in the F2 progeny remember you are trying to distinguish between sex linked and autosomal traits so you will have to include sex as a phenotype 3 wild type 39 1 white eyed for both males and females e null hypothesis which describes the expected results for this experiment Something along the lines of The white eye trait is determined by two linked autosomal and recessive genes and so will show a 3 wild type 39 1 white eyed ratio in the F1 generation 4 How would the expected ratios of progeny change compared with 3 if the two mutations are unlinked on different chromosomes What if the mutations are far away from each other on the same chromosome so that recombination is possible In the germline of which ies would recombination occur If unlinked and on different chromosomes then the traits will segregate in a 988 ratio of wild type brown eyes bright red eyes white eyes If they are on the same chromosome there should be more than 91 6ths wild type and more than 1 6th white eyed ies but fewer than 3 16ths for each of the brown and bright red eyes Recombination will occur in ANY germline but the recombination of importance happens in the F1 adult ies They are the heterozygotes and as they make gametes their chromosomes can experience crossing over Crossing over in the P generation does not change the chromosomes since they are homozygous and we usually don t care what happens past the F2 unless the inheritance patterns are particularly muddled by other factors PV92 PCR INFORMATICS LAB DATA ANALYSIS Answer the following questions based on the data collected from your group s gel 1 Lanes 36 are the controls for this experiment What was in the PCR tube for each control and what type of control is it What do the results seen in the control lanes tell you about the validity of the experiment The tubes containing DNA were the positive controls One had DNA with the Alu insert one without and one with both types of DNA The tube containing water was the negative control A valid experiment should have bands in the positive controls to show that the PCR worked correctly while the negative controls should have no bands to indicate that there was no outside or cross contamination of the PCR mix 2 It is often helpful to think about the independent dependent and controlled variables for an experiment The independent variable is the one aspect of the procedure that is intentionally altered for each sample by the researcher The dependent variable changes in response to a change in the independent variable Controlled variables are all other aspects of the experiment those kept constant for all samples What are the independent dependent and controlled variables for this experiment I don t think this is helpful at all for this type of assay Dr Bushart The independent variable is the DNA of the students either from the perspective of the DNA in the cell or from the perspective of the isolated sample in the tube The dependent variable would be the banding pattern produced from the PCR and gel The controlled variables are the conditions PCR program and the materials used master mix 3 observed genotype frequency 33125 0264 65125 052 19125 0152 4 allele frequency p q 1 33 X2 65125 X2 0524 19X2 65125X2 0412 5 p2 2pq q2 1 6 You can calculate using 35 answer


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