New User Special Price Expires in

Let's log you in.

Sign in with Facebook


Don't have a StudySoup account? Create one here!


Create a StudySoup account

Be part of our community, it's free to join!

Sign up with Facebook


Create your account
By creating an account you agree to StudySoup's terms and conditions and privacy policy

Already have a StudySoup account? Login here


by: Ezequiel Orn


Ezequiel Orn
GPA 3.89


Almost Ready


These notes were just uploaded, and will be ready to view shortly.

Purchase these notes here, or revisit this page.

Either way, we'll remind you when they're ready :)

Preview These Notes for FREE

Get a free preview of these Notes, just enter your email below.

Unlock Preview
Unlock Preview

Preview these materials now for free

Why put in your email? Get access to more of this material and other relevant free materials for your school

View Preview

About this Document

Class Notes
25 ?




Popular in Course

Popular in Biology

This 34 page Class Notes was uploaded by Ezequiel Orn on Sunday September 6, 2015. The Class Notes belongs to BIO 366 at University of Texas at Austin taught by Staff in Fall. Since its upload, it has received 13 views. For similar materials see /class/181730/bio-366-university-of-texas-at-austin in Biology at University of Texas at Austin.




Report this Material


What is Karma?


Karma is the currency of StudySoup.

You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!

Date Created: 09/06/15
e Express on m CH Figure 1221 m y 9 m m Hmh vTVUGA a Termiuaw hairpzn structure AUG 39 Pause sunnure A Alltmallve RNA secondary slructum of the eadcr ranxript paw Termination unfurmalicn Leader peptide UUUUUUU codmg regmn RNA AUG 39 Tmmaipuon Fm DNA a 1 2 3 4 mum gene Prcmcer ATC w 4 TEA Leader pep de A v J codmg region mime Amnuamr egulation of Gene Expression m Figure 1222 Global Regulatory Mechan39sms m Table 1 31 mame mmwgmm uanan mugmm 5mm nmlmnw Dimml m quotmum mmugmm mmummm um WWWWHW upmrmuumum whmnnvjmmmm wannrmmm mm m WWW cmnmm mmww wmmmm Mmmzm mnwml wn mmmm xmnmw WW me mm nummmm www 1 Wu nmwmmmm mm mum Wm WM mrgwm mm wmmmn mama me mmnmm nwmdmw Mmmmnu wwmwmmm 4m anthrax e WWW WM undamme quotWWW mm mquot M mum munIVny Wmmmm mum ngummf Mmul qm n quotWNW lmmwmw Jmmm mm MWMW 0qu mnquotx Mm mumwmmu nmwmmq mmm mmnw wwwmn quotMIAMI Yu zmwwmmyuuu mummumm kmmymmh um nmqm mm nm m thxIpum pil thmunwmnmu mmwcxmmmynmv M MUWW qunX 9 wa Hm quotzmmwmm ummvmwwnu MWquot nmmqm mum wryWE mm mm 1 WM Mm mm human 0me lemqmunm MW Rummy Figure 131 5 b 3 5 01 I 0 X in m 2 o a 4 O 001 mum nan p Wmam unnu m Ivampnmd pymvau HIM Figure 1 33 70 755 735 40 1 A 7 r r GTCAGTTAGCTCAC RNA Pu inleratticn raglan CAC TCALI39CGAGTG CAPAbindw39ng Sile B Class promoter Class H promoter mm Figure 1 34 RNA Polymerase Global Regulatory Mecha I1 2 l 10 hexamer 735 hexamer Global Regulatory Me Figure 135 m m mwlacnam E mm A mg 7 W W m a l MunEm n 39Km Jug I r H mm Table 1 31 TABLE 13 Some E coliglobal regulatory systems C Growth limitation Stringent response Stationary phase Oxygen Stress Osmoregulation Oxygen stress le Shock pH shock S orthophosphate P Response to lack of sufficient minoacylatedrlRNAS for protein synthesis protection esponse to anaerobic environment osmotic upshift Adjustment to osmotic environment Protection against reactive oxygen species Tolerance to abrupt temperature increase Tolerance of acidic environment reA RelA SpoT SpoT rpoS RpoS fnr Fnr arcAB AmAB k n F Ikrlnn krlnn rpoS RpoS mlCF soxS SoxS oxyR OxyR rpoH RpoH rpoE RpoE cadC CadC Regulatory genes Category of Some genes and System Response proteins mechanism operons regulated A Nulrlenl Ilmlm on Carbon Ir n I II A rizp or lac am gal mi and numerous repressor other c Sioume operons ntroi of cm frilR CRA DNArbinding activator Enzymes of glycolysis fermentative vs or repressor iltrebs cycle oxidative metabolism Nitrogen Response to ammonia rpoN NtrA Sigma factor o5 griA GS and operons for amino limitation acid degradation rifch NtrBC Tworcomponenl system Phosphorus tarvau39on for inorganic phoBR PhoBR Tworcomponenl system gt33 genes including phoA 39 pppGpp metabolism Sigma factor o CAP family of DNA7 binding protein Tworcomponenl system Sigma factor 5 Antisense RNA Arac family of DNA7 binding proteins LySR family of DNA7 binding proteins Sigma factor 92 Sigma factor oz ToerrelaIed DNA7 binding protein bacteria alkaline phosphatase and psf operon P uptake rRNA NINA gt40 genes including ofsBA trehaiose synthesis and tips DNArbinding protein gt31 transcripts including narCHl nitrate reductase gt20 genes includin cob s cobalamln synthesi kdpFABC K uptake system gt16 genes including oSmB an outer membrane lipoprotein membrane porins ompF porin gt10 genes including sodA Superoxide dismuiase and mlCF antisense RNA regulator of ompF gt1 0 genes including kafc catalase gt20 genes encoding Hsps heat shock proteins including driaK dria an gr E chaperones and on cpl cpX and hfB proteases gt10 genes including rpoH on and degP a periplasmic protease Many genes including cadBA amino acid decarboxylase Optical density 0 39I Galactose Glucose Time Figure 1 31 L O I I I I X C O Global Regulatory Mechanisms Figure 1 32 A Z Ln use Inactive adenylate cyclase LgtL Jlun m eI vl 391 ll Lily nlyxb U PEP gt pyruvate Hprwp H 9 Inactive 2 Mpermease La Ctose n 0t tra nspotted Active adenylate cyclase H pr PEP gtC pyruvate Hprwp Ldl imp Global Regulatory Mechanisms Figure 1 33 A 70 55 35 1 0 1 C T C AC T T AC C T CA C 39RNA Pol interaction region39 CACTCAATCGAGTG IgtmRNA CAPbinding site B Class promoter Class II promoter Figure 1 34 RNA Polymerase I2 I 1 O hexamer 35 hexamer Global Regulatory Mechanisms La ctose 6 lucose La ctose H luuy e Lactose HWII now Lactose Glucose Figure 1 35 RNA Pol interactions CAP site Operator l l l m m 100 90 80 70 60 50 40 30 20 10 1 10 20 30 40 35 region 10 region CAP RNA Pol I lacZYA mRNA Repressor l l CAP Repressor l l ll J Global Regulatory Mechanisms Figure 1 36 CAPsite 10 1 35 I I 1 GTCACTTACCTCAC I I TATCTTCTGTCCAATT I CACTCAATCCACTC I I ATACAACACACCTTAA L81 TUVS A AA T Tl Class Class II Class III Global Regulatory Mechanisms Figure 1 37 NH3 glutamate gt glutamine Glutamlne synthetase Glutamine ocketoglutarate gt 2 glutamates Glutamate synthase High NH3 NH3 xketoglutarate gt glutamate Glutamate dehydrogenase NH3 glutamate gt glutamine Glutamlne synthetase Figure 1 38 NtrB gt NtrB39V P 140 108 1 Opencomplex promoter 5 Global Regulatory Mechanisms A Tworcomponenl system Penplasm Cytoplasm Semor domam B Phosphorelay N DNArbmdmg domam DNAV doma lndmg m Box 133 C SpoOBSpoOF cocrystal structure SpoOF SpoOB dimer SpoOF binding domain p1 p 2 gInA p3 ntrB ntrC Global Regulatory Mechanisms Figure 1 310 670 promoter 35 l 0 1 A gt TTGACATATAAT T AACTGT ATATTA 54 promoter 24 1 2 1 A gt CTCCXATTGCA T GACCXT AACGT G32 promoter 1 35 10 A TCTCXCCCTTGAAjECCCCATXTA T AGAGXGGGAACTT GGGGTAXAT Box 1 34 N A HTH C 477 amino acids Closed complex NTP Activator C NDP Pi Open complex 394 Global Regulatory Mechanisms Table 1 32 TABLE 132 Genes for nitrogen regulation Gene Alternate name Product Function glnA Glutamine synthetase Synthesize glutamine glnB P PHUMP Inhibit phosphatase of NtrB activate adenylyltransferase glnD Uridylyltransferase Transfer UMP to and from P UTaseUridylylremoving enzyme UR glnE Adenylyltransferase ATase Transfer AMP to glutamine synthetase glnF rpoN 03954 RNA polymerase recognition of promoters of Ntr operons ntrC gInG NtrC NtrCP04 Activator of promoters of Ntr operons ntrB gInL NtrB NtrBPO4 Autokinase phosphatase phosphate transferred to NtrC Global Regulatory Mechanisms Figure 1311 A Transcriptional DNA Regulation activation binding l l l NI m C 1 ATPbinding 459 Amino acids motif B gt 4 GCACCANgTGGTGC CGTGGTNgACCACG NtrC binding quot binding I gnA ITTCCCACAGATTTCCCTI 440 108 39 27 11 1 UAS Promoter gnA 1 Global Regulatory Mechanisms game Table 1 33 Phenotypes of envZ and ompR mutations Genotype Phenotype envZ ompR OmpC OmpF envZ ompRi OmpC OmpF envZnull ompR OmpC OmpF envZ ompR2C0n OmpC OmpF low osmolarity envZnull ompR2C0n envZ ompR3C0n envZ ompR3ConenvZ ompR OmpC OmpF high osmolarity OmpC OmpF low osmolarity OmpC OmpF high osmolarity OmpC OmpF low osmolarity OmpC OmpF high osmolarity OmpC OmpF low osmolarity OmpC OmpF high osmolarity indicates that OmpF levels are reduced but not eliminated Global Regulatory Mechanisms Box 1 35 A Negative regulation B Positive regulation I DsrA RNA I DsrA RNA 3 5 5 3 3 1 7 3 L 3 2 Base 3 3 A 5 pairing 3 5 5 SD 5 rpoS mRNA on h e rpoS gt ll OxyS RNA ll OxyS RNA allowed 5 Titrates 5 5 Base V pairing S gt on SD rpo rpoS mRNA Translation allowed 5 fhlA mRNA Global Regulatory Mechanisms Figure 1 312 dth tox lt 5 phage gt fQN gt I I dth tox Global Regulatory Mechanisms Figure 1 31 3 l l39 l Outer membrane Periplasm Tox R Inner 1 a ll membrane lllllll ll 1 orin ornpU Virulence 399 factors k V cm Val hr sqen blunt VPI Cytoplasm toxR OX5 Irpl lz pl ILpAJ a Global Regulatory Mechanisms Figure 1 314 bng bng bng L r bng 666666 CCCCCC ngs 00 bng CCCCCCC CCCCCCC ngs C C bng CCCCCC 9 Global Regulatory Mechanisms M l P Iquot lil IlmH 7 39 II39IRNA Z Z O O 4 4 Denatured 5 5 proteins quot V Figure 1 31 5 L3 397 4 0 II Ill 391 Global Regulatory Mechanisms Figure 1 316 r r r IthrUI tyrUI egTI thrTI tufBI I rpKI rpAI rpl I rpL IrpoBlrpoCI Figure 1 31 7 mRNA 39 One copy Two copies T T T Gene A Gene A Gene A One copy Two copies Global Regulatory Mechanisms Figure 1 319 Ribosome sta lls pump 391 71 1 RelA makes PPG PP


Buy Material

Are you sure you want to buy this material for

25 Karma

Buy Material

BOOM! Enjoy Your Free Notes!

We've added these Notes to your profile, click here to view them now.


You're already Subscribed!

Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'

Why people love StudySoup

Jim McGreen Ohio University

"Knowing I can count on the Elite Notetaker in my class allows me to focus on what the professor is saying instead of just scribbling notes the whole time and falling behind."

Kyle Maynard Purdue

"When you're taking detailed notes and trying to help everyone else out in the class, it really helps you learn and understand the I made $280 on my first study guide!"

Bentley McCaw University of Florida

"I was shooting for a perfect 4.0 GPA this semester. Having StudySoup as a study aid was critical to helping me achieve my goal...and I nailed it!"


"Their 'Elite Notetakers' are making over $1,200/month in sales by creating high quality content that helps their classmates in a time of need."

Become an Elite Notetaker and start selling your notes online!

Refund Policy


All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email


StudySoup has more than 1 million course-specific study resources to help students study smarter. If you’re having trouble finding what you’re looking for, our customer support team can help you find what you need! Feel free to contact them here:

Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to

Satisfaction Guarantee: If you’re not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.

Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.