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Genes & Gene Expression

by: Charity Daniel

Genes & Gene Expression BIS 101

Charity Daniel
GPA 3.76

Carlos Quiros

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Carlos Quiros
Class Notes
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This 2 page Class Notes was uploaded by Charity Daniel on Tuesday September 8, 2015. The Class Notes belongs to BIS 101 at University of California - Davis taught by Carlos Quiros in Fall. Since its upload, it has received 20 views. For similar materials see /class/187624/bis-101-university-of-california-davis in Biological Sciences at University of California - Davis.

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Date Created: 09/08/15
TRANSDUCTTON PROBLEMS The following data were obtained by Charles Yanofsky in a 1959 study of the structure of the tryptophan operon region of E coli In your analysis of these data take note of the fact that Yanofsky knew from other data that the cys gene was at one end of the try operon and that the genes of the operon all had to be on one side of cys Donor Recipient Selected Unselected Cotransduction of Genotype Genotype Marker Marker Unselected Marker with cys cys trpE cys trpE cys trpE 63 cys trpC cys trpC cys trpC 53 cys trpA cys trpA cys trpA 46 cys trpB cy trpB cys trpB 47 Determine the gene order of the trp operon genes based on analysis of the cotransduction frequencies 2 A later transduction analysis of the trp operon using different donor and recipient genotypes was used to verify the map created by Yanofsky This genotypes was used to verify the map created by Yanofsky The genotypes in this transduction analysis are Donor cys trpB trpA X Recipient cys trpB trpA The cotransduction data are Recombinant Genotypes Observed Number cys trpB trpA 139 cys trpB trpA 18 cys trpBtrpA 141 cys trpB trpA 4 302 Do these data confirm or refute the gene order identified from analysis of he Yanofsky data Explain Explain the recombination events that have occurred to create each of the four classes What are the cys trpB and cys trpA cotransduction frequencies Answers 1 Most likely order is cys E C B A higher the co transduction shorter the distance of the trp gene to cys Order of B and A tentative since cotranduction frequencies with cys are very close Crossover region Crossovers in the following regions will explain the expected genotypes Recombinant Genotypes Cross overs Observed Number cys trpB trpA 1 and 4 139 cystrpB trpA 1 and 3 18 cystrpBtrpA 1 and 2 141 cys trpBtrpA 1 2 3 and 4 4 302 Only even number of crossover possible Single or other odd number of crossover will break the circular chromosome from recipient Least frequent class id quadruple crossover Expected from product rule of probability 2 c Cotransduction frequencies cys and B 13918302 052 cys and A 1394302 047 2a This experiment confirms results of the first one for genes B and A The gene order is cys B A since cotransduction of cys B is higher so it is closer to cys than cotransduction of cys A it is farther from cys Note that cotransduction freq is the opposite to recombination freq Here we are determining the frequency of two parental alleles staying together Also note that we are ignoring the position of C and E since these were not tested in the second experiment


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