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This 2 page Class Notes was uploaded by Renee Lehner on Wednesday September 9, 2015. The Class Notes belongs to BIOL 200 at University of Washington taught by Staff in Fall. Since its upload, it has received 16 views. For similar materials see /class/192333/biol-200-university-of-washington in Biology at University of Washington.
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Date Created: 09/09/15
DNA replication 1 DNA deoxyribonucleic acid the hereditary material of cells and certain viruses Why was protein believed to be the genetic material of cells Bacteria and their viruses were the experimental systems used to study macromolecules Koch s Postulates are used to prove that a disease has a microbiological origin What are these steps Why did Griffiths experiment suggest that DNA was the genetic material of cells 0 Avery MacLeod MacCarty demonstrated that transforming principal was DNA What was the concern raised by advocates of the protein theory of Avery s work 0 Hershey and Chase experiment why did Hershey and Chase use phage T2 to demonstrate that the genetic material was DNA How can one distinguish between DNA and protein based on their chemistry 2 Chemistry of nucleic acids DNA and RNA 0 Are polymers of nucleotides 0 What are the component parts of a nucleotide and how does one nucleotide differ from another 0 What is the difference between a nucleoside and a nucleotide o Nucleotides are covalently joined through a phosphodiester bond Where is this linkage formed between nucleotides 0 What is meant by the 5 and 3 ends of a polynucleotide strand Most cellular RNAs are single stranded ssRNA and all cellular DNAs are double stranded dsDNA 3 DNA secondary structure 0 A xihelix structure 0 What is meant that strands are of opposite polarity or antiparallel o How do bacteria and eukaryotes differ in the structure of their chromosomes and how to bacteria and eukaryotes condense their DNA so it can be packaged fit into the cellnucleus 4 DNA replication Replication occurs by the semiconservative model of replication How did the Meselson and Stahl experiment distinguished between the 3 models proposed for how DNA replication might occur Role of DNA polymerase DNA pol How are DNA pol III and I used in replication Synthesis always proceeds in the 5 to 3 direction What does this mean DNA pol can only add nucleoside triphosphates to a free 3 OH group Can DNA pol initiate DNA replication on its own o In bacteria replication starts at a speci c site and only at this site called the origin of replication or OriC site 5 Model for DNA replication in bacteria Theta Model a Role of initiator proteins helicase and single stranded binding proteins b Replication is bidirectional c Synthesis of RNA primer d Synthesis is continuous for one complementary strand and discontinuous for the other What are Okazaki fragments Function of DNA ligase and topoisomerases Termination of replication at Ter site Tus protein g Modi cation of certain DNA bases following replication methylation of certain adenosines h Replication is not an error free process 139 4 Replication in eukaryotes o replication is initiated at several but speci c sites 0 What is the End Problem 0 How do certain bacteria with linear chromosomes replicate the ends of there genomes
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