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by: Anahi Sawayn


Anahi Sawayn
GPA 3.86


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Class Notes
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This 13 page Class Notes was uploaded by Anahi Sawayn on Saturday September 12, 2015. The Class Notes belongs to CrmLaw 20 at University of California - Irvine taught by Staff in Fall. Since its upload, it has received 63 views. For similar materials see /class/201847/crmlaw-20-university-of-california-irvine in Criminology, Law And Society at University of California - Irvine.

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Date Created: 09/12/15
Crystallography Recap and Review l Co ncepts review I Crystallization Forming single crystals means inducing a phase change from a solution phase to a solid phase Sometimes solvent is trapped in the lattice which means we have to account for it in the structure solution if it has an ordered structure regular position Protein crystals always have solvent anywhere from 2080 Crystallization Methods Data Evaporation Recrystallization from selected solvent Batch crystallization under oil drives salt or protein from the metastable region ofthe phase space Vapor diffusion hanging or sitting drop Microdialysis Free interface diffusion microfluidic crystallization chips Batch crystallization mix everything and let it sit collection Mount the crystal Center the crystal Collect a matrix to determine the unit cell parameters and index the diffraction pattern Scale the data Reduce the data by applying any symmetry Submit the data to structure solution solve the phase problem I Solving the phase problem is like the art of decoding an encrypted message the diffraction pattern is the picture of a molecule in secret code Batch crystallization of salts saturated NaCI Terazakitype microtiter plate crystal Crystals EL The Classic HDVD WWI The Variant Micro SD D Batch Interface Micro DiaIVSiS Diffusion Multilayer soft lithogra hy w Semen m a dam shwng c E It n I g WWW 3 ad W I 3 MW 3 L o am 28358 4 m ms 322 i 0 32 i ai Ham 5 I I I I Frequency Concentration ln mM E Rubberlike material which deflects under r pressure to create an mng a l new wmmm effective seal quotmum m r mm Wm M l htt www uidi mcom mmpm u muslmm nano exhtm Mb 5 mquot W Coded reciprocal space image of molecule Decoded real space image of molecule Cracking the crystallographic code Ms diffraction physics and reciprocal space are intensity statistics and distributions probabilities outliers symmetries phase relations bootstrap prior knowledge sequence physical data chemical knowledge 39 The art of code breaking 4 the nature of the code 1917171914 20231819 8 121619 8 3 21 8251814 18 6 318 8 1518221811 2 lt N lt 26 Probably letters a secret message 1019 possibilities of random combinations Statistical analysis 12 3 4 5 6 7 8 B1011121314151617181920212223242526 39 The art of cede breaking data analysis 12 3 4 5 6 7 8 B1011121314151617181920212223242526 1917171914 2023 E19 8 121619 8 3 21 825 E14 E6 3E8 15E22 E11 19 17 17 19 14 21 8 25 E 14 E 6 Pattern recognition and statistics A 17 17 A 14 2o 23 21 8 25 E 14 E 6 A T T A c K 23 21 8 25 E c E 6 20 23 E 19 3 E 12 16 19 8 3 15 E 22 E 11 12 3 4 5 6 7 8 B1011121314151617181920212223242526 12 16 A 8 3 15 E 22 E 11 MuItIsolutlon methods 12 16 A 8 3 15 E 22 E 11 Substructure 19 17 17 19 14 21 8 25 18 14 18 6 19 21 19 17 37 19 14 20 23 21 8 25 18 14 18 6 20 23 18 3 19 18 16 25 19 18 8 12 16 19 8 3 8 15 18 22 18 11 19 14 2O 23 18 19 18 14 18 6 3 18 8 12 16 19 8 3 8 15 58 22 18 11 8 8 12 16 19 8 3 15 19 22 18 11 39 The art of code breaking 4 context and restraints ATTACK23E1981216A83 21825ECE6 3E8 15E22E11 Context 60 years ago on a pacific radio intercept station SE of Midway or we we mne an M an mm M SSW mlquot tun lt1039 MN 211 1 Today s activities Part 1 I Crystal inspection l l Observe the lysozyme batch crystallization i l Note which salt gives the best crystals l l Best is relative Ideally you want larger single crystals that are well formed have clearly defined shape edges l l Caveat salt crystals can sometimes fool us into believing we have protein crystals Protein crystals have different physical properties soft not brittle due to high solvent will soak up small molecules like ligands drugs and dyes I The dye test you will pick a food coloring of your choice and add a small amount to one of the tubes of lysozyme crystals you have grown Observe over time If you have protein crystals they will take up the dye eventually becoming darker than the immediately surrounding solution Yureka


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