INTRODUCT BIOCHEM BCMB 3100
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This 3 page Class Notes was uploaded by Adah McCullough III on Saturday September 12, 2015. The Class Notes belongs to BCMB 3100 at University of Georgia taught by Staff in Fall. Since its upload, it has received 29 views. For similar materials see /class/202194/bcmb-3100-university-of-georgia in Biochemistry and Molecular Biology at University of Georgia.
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Date Created: 09/12/15
contammg Order of Chemical Reactions FirstOrder Reaction Rate depends on S Substrate Product V0 initial velocity k1 forward rate constant substrate Second order reaction Rate depends on both s1 and s2 S1 s2 69 p1 p2 v0k1s1s2 Glucokinase example rate depends on glucose and atp Glucose atp look at powerpoint Turn it into a quotpsedofirst order reaction then can determine rate constant HOW TO 1 Measure formation of product at different substrate 2 seepowerpoint 3 Plot V0 against S 4 slope is forward rate constant k1 v0 k1 s Rate constant how rate changes with changes in substrate What does it mean to have a large rate constant The larger the rate constant the faster the reaction is performed Large change in initial velocity with a relatively small change in substrate What does it mean to have a small rate constant The smaller the rate constant the slower the reaction is performed Large change in substrate with small change in initial velocity Keep adding substate reach a plateau for initial velocitywhy Equation for rectangular hyp erbola y powerp oint What is 39b E S 69 ES 9 E P MichaelisMenten equation v0 vmaXSb S Is it the association constant or how easily ES forms klk1 ethat idea was proposed and didn t make sene Is it the dissociation constant or how easily ES falls apart K1k1 llow easily ES falls apart k1 should be higher and b will be smaller and in turn v0 will be higher But how else can ES fall apart K1 k2k1 KM all of that equals b KM is an indicator oh how stable ES is ROUGH MEASURE OF AFFINITY ES 9ES9EP KM pp If theres a large K1 then ES will form readily and you only need a small amount of substrate Not so much substate is needed to drive the reaction forward KM the smaller the km the more stable the ES compleX is If K1 is much smaller and can t form so easily there is a weak interaction ES is less stable and A LOT of substrate is needed to drive the reaction forward KM will be larger How an enzyme is doing what it is doing KM and VMaX More activity from more enzyme and lower KM More enzyme NO effect on KM and Vmax max rate you can produce product will increase and lower blood glucose levels If the enzyme is doing a quotbetter job KM will be smaller Vmax will not change Blood glucose levels will be lower because KM is doing a better job of getting the glucose out Product of this reaction is phosphorylated glucose Second experiment activity of purified glucokinase from wildtype mice control and seven different I nouse lines Activity KM Vmax Blood glucose Control 6mM 100 units mg Normal 6mM 500 Higher expression hyp oglycemia 15 mM 113 Hypoglycemia binds at lower concentration of glucose 12 mM 300 quotbad enzyme Not as functional Needs more substrate to drive reaction More enzyme Hard to know blood glucose level lChU39lvF 14 mM 150 unitsmg REALLY SHITTY ENZYME SUCKS BALLS Hyperglycemia Need more glucose to bind for activity Finishing the chart at the breakout High vmaX cant come
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