Popular in Biochemistry I
Popular in Biochemistry
BCM 475 - M001
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This 2 page Class Notes was uploaded by Stephanie Morales on Sunday September 13, 2015. The Class Notes belongs to BCM 475 - M001 at Syracuse University taught by M. Braiman, R. Welch in Fall 2015. Since its upload, it has received 33 views. For similar materials see Biochemistry I in Biochemistry at Syracuse University.
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Date Created: 09/13/15
September 8th How does immune response work It is innate and adaptive Cellular barriers it is an innate immune response It includes white blood cells polymorpho barrier monocytes and macrophages elevated temp ect Hair like projection in cells a lot of area used to present things Humoral immunity antibodies B cells produced by bone marrow Cellular immunity specific T cells they make cytokines Adaptive immune response they recognize specific molecules and recognizes antigens Innate natural immune response responds to non self cell surface structure It is non antigen specific They respond either by absence of a password or foreign object Innate natural immune response has no immunological memory and can be induced by cytokines It doesn t use Tcells or antibodies It is however involved in inheritant disease and neoplastic diseases Examples of an interaction between innate and adaptive is macrophages making cytokines when it sees antigens Macrophage ingested pathogens and as it is eating it presents it to T cell to get help to fully attack the antigen September 10th CD 4 amp CD 8 are code receptors that bind to antigen as T cell binds to antigen Polymorphonuclear leucocytes have granules which can cause overproduction Basophils are involved in allergy NEED TO KNOW HOW TO IDENTIFY EACH TYPE OF CELL It is important for a cell to make itself before it attacks because if he doesn t there wont be any of itself left Myeloid is found in the blood stream Herd immunity means that it is protected by immunity of everyone else In uenza Type A happens to people and animals The worst one also known as bird u because it comes from wild birds It is constantly changing Type B amp C are both found in humans only are not as bad as Type A There are different vaccines from different times a year H5N1 H is hemaglutin variant amp N neuraminidase variante September 9th 4 category of structure primary linear secondary local structure that has peptide bonds It allows maximization of conformation tertiary 3D arrangement Has a helix and beta sheets quaternary multi subunit protein tertiary structure separate hydrophilic and hydrophobic is inwards Membrane proteins are integral and peripheral bateriorhodspin is a membrane of a helices It has 7 helices the interior have to be nonpolar interior space of a pore is hydrophilic and bacterial porin is built of beta strands The R group facing porin is hydrophilic determine if protein is transmembrane by the sequence The residues are usually nonpolar and uncharged most are a helices hydrophathy plot estimated free exams change for 20 amino acid stretch glycophorin indicates single hydrophobic alpha helix It is identified by the number of peaks Linear sequences fold into domain having independent globular structure which are connected by exible linkers Quaternary structure has subunits it may be identical or distinct amino acids The structures are denatured by urea and guanidium chloride Protein disulfide bonds can be broken by addition of excess beta Cooperativity low probability 50 folded and 50 unfolded Protein chooses to be either completely unfolded or folded September 11th Progressive stabilization of intermedites has 42 Kjmol free energy it is favorable in the folded state If proteins would have to fold by chance it wouldn t happen They form intermediates Proteome is functional representation of the genome 50000 genomes sequenced between eukaryotes 2500 and prokaryotes determine protein by sequence fucniton and structure Protein purification needs to have an assay to see if it is possible Specific activity the amount of property per unit of mass Purest protein has highest activity in native state Separation by size size of pores by dialysis Large proteins are retained However in the size exclusion chromatography the big proteins are the first to come out Separation by charge column chromatography you add non charged salt which increases the ionic strength and disrupts interaction between charged proteins and columns Electrophoresis unfolds proteins by making it snake through the gel Larger molecules take more time
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