Principles of Biology II
Principles of Biology II BIOL 1108
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This 6 page Class Notes was uploaded by Greyson Gutkowski on Thursday September 17, 2015. The Class Notes belongs to BIOL 1108 at University of Connecticut taught by Donald Les in Fall. Since its upload, it has received 22 views. For similar materials see /class/205864/biol-1108-university-of-connecticut in Biological Sciences at University of Connecticut.
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Date Created: 09/17/15
Oluwasegun Afolayan Section 3 Photosynthesis Lab Assignment 10122010 Ah am my a M 1 5674 Elli Key Red Line Leaf m o Wavelenyii rim Figure 1 Absorption Spectra of Leaf Pigments Blue Line Yellow Petal Green Line Red Cabbage Abmmmn a no nm All a NH 1253 Key Blue Line Tube 1 Nu 10D mom rm um Figure 2 Hill Reaction Light vs Dark Green Line Tube 2 Yellow Line Tube 3 Purple Line Tube 4 mummu 1 mm quotm n n i U 10 IUD 1110 REM 451 Email mammx Figure 3 Effect of Color Acetate filters on the Hill Reaction Key Red Line Clear Acetate Tube 5 Blue Line Red Acetate Tube 6 Green Line Yellow Acetate Tube 7 Yellow Line Green Acetate Tube 8 Purple Line Blue Acetate Tube 9 Answers to Questions Page 53 1 Iquot S P Leaf Extract Purple Blue and Red are absorbed while Green and Yellow are transmitted Yellow Petal Extract Purple and Blue are absorbed while Green Yellow and Red are transmitted Red Cabbage Extract Purpe Green and Yellow are absorbed while Blue and Mainly Red are transmitted Leaf Extract The Peak Absorbance is 08 7max at a Wavelength of 225nm Yellow Petal Extract The Peak Absorbance is 067 7max at a Wavelength of 235nm Red Cabbage Extract The Peak Absorbance is 098 7max at a Wavelength of 75nm Yes it does I think so because the other chlorophyll types c and d are present only in some algae The other accessory pigment which I know is present carotenoids work with both chlorophyll a and b Accessory pigments are light absorbing compounds found in photosynthetic organisms that work in conjunction with chlorophyll a They absorb light and transfer that light energy to the photosystem chlorophyll Some of these accessory pigments particularly the carotenoids also serve to absorb and dissipate excess light energy or work as antioxidants Page 54 l 2 In the quotlightquot treated Coleus leave there is no change and the leaf has its normal green pigment On the other hand in the quotdarkquot treated Coleus leave the base part ofthe leaf blade the part closer to the stem is stained purple which shows the presence of starch Ithink this is because is being transported to the other parts of the plants so it is going to pass out through the leaf stem ie it moves closer to the base ofthe blade The red pigment is water soluble while the green pigment is alcohol soluble Page 64 1 Iquot S P We set the spectrometer to absorb at 620nm because the orange color the roughly between the main colors involved in photosynthesis ie red yellow green and blue and also because none of our acetates were colored orange This served to ensure that there was a more accurate reading of the data Yes This can be seen in Tubes 2 amp 3 In Tube 2 there was DCPIP but no chloroplast so there was nothing to reduce the DCPIP hence no reduction Vice versa in Tube 1 Meanwhile in Tube 3 both were present hence there was a reduction of DCPIP Yes This can be seen in Tubes 3 amp 4 In Tube 3 there was light involved hence the result was a reduction in DCPIP Meanwhile in Tube 4 the reaction took place in the dark ie without light and there was no reduction in DCPIP In the filtering part of the experiment we can see that the reduction rate of DCPIP reduces over time Also I think that the reduction rate when based on the different colors of acetate is based on the Absorption Spectra of the Chlorophyll and that the amount of reduction is based on whether the color will be highly or lowly absorbed or transmitted n Clear Acetate There was a very low reduction rate This could be because clear white light is highly transmitted Red Acetate Here there was a moderate reduction rate because red is moderately absorbedtransmitted Yellow Acetate There was a very high and in fact the highest reduction rate because there is a very high transmission of yellow light Green Acetate Again the reduction rate is high and the rate oftransmission is also high Blue Acetate In this there is low reduction of DCPIP because a lot of blue light is absorbed
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