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by: Robert Notetaker
Robert Notetaker

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Regulatory Regions. DNA and processes of on an off genes.
DNA: Identify and Disease
Class Notes
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Popular in DNA: Identify and Disease

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This 2 page Class Notes was uploaded by Robert Notetaker on Saturday March 12, 2016. The Class Notes belongs to at Brigham Young University - Idaho taught by in Winter 2016. Since its upload, it has received 7 views. For similar materials see DNA: Identify and Disease in Biology at Brigham Young University - Idaho.


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Date Created: 03/12/16
Restriction Enzymes Facter 8 Plasmid Vector cut open by a digest to create sticky ends(Sticky ends are sticky, use the same cutting enzyme)  E. Coli E. Coli will reject gene by itself. Has to be circular. E. Coli needs a regulatory region. Region deals when where and how much of the protein to make. Focus: How do the regulatory regions work. Proteins that control transcription(Transcription factors) Transcribe mRNA translated- protein Activator binds only to skin specific: Activator binds only to neuron specific enhancer. Regulatory region DNA sequences: Enhancer, Core Promoter, Silencers Proteins that bind to regulatory Region: Activators, GTF’s + RNA Polymerase, Repressors Repressors are Like the brake Pedal. How does skin know to become a skin cell? Repressor comes from DNA. Cell has a specific gene. So there would have to be Organizer cell: Skin Cell Receives the signal and Organizer Cell gives signals to the skin cell but too far away from the neuron cell. Neuron is waiting for a signal and the signal Enhancer Core Promoter Operator(Silencer) cAMP Activator RNA Polymerase Laci Laz, Lac Y, Lac A


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