MOLECULAR GENETICS PCB 4522
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This 2 page Class Notes was uploaded by Cameron Koss I on Friday September 18, 2015. The Class Notes belongs to PCB 4522 at University of Florida taught by Staff in Fall. Since its upload, it has received 6 views. For similar materials see /class/206952/pcb-4522-university-of-florida in Biology at University of Florida.
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Date Created: 09/18/15
Exam 1 lecture 3 review questions 1 What is a compatibility group 2 Can two incompatible plasmids belong to the same compatibility groups 3 How does the ColE replicon control the copy number What is the size of the RNA primer What is the structure of the RNA primer Which enzyme is needed in order to generate the 3 hydroxyl What is the persistent hybrid 4 In the ColE Replicon what acts as positive and negative controls 5 How can antisense RNA coun plasmid copy number 6 How can a cell generate a new compatibility group 7 Where can you nd an ARS 8 How many sequence domains are present in the ARS 9 What characteristic feature of the sequence aids in melting 10 List the first step in ARS recognition What other proteins can bind to the ARS and where Does ORC formation require ATP Is there any redundancy in the yeast ARS 11 Does the ORC bind to the origin through the cell cycle or does it leave the origin if so when 12 List two other proteins that are important for ARS function Do they stay or leave at some point during the cell cycle 13 Describe the role of Cdc6 in the recruitment ofthe Mcm complex 14 What comprises the licensing factor What is its function 15 What is the best system to study licensing factor controls and why 16 Why must the nuclear membrane be permeabilized in order for licensing factor to function 17 Where can you find Dloop type replication 18 Is it possible to separate in time replication of each stand of a circular DNA If so give an example 19 Where is the origin of replication located for synthesis of the new L strand inew H strand 20 What special nuclease is required in generating a primer for Dloops What must it be able to do 21 List some differences between oriC and Dloop replication 22 Know the new de nition of the origin of replication 23 List solutions to the problems inherent in the replication of linear DNA 24 How does adenovirus prime top strand singlestrand replication Can the initiation start anywhere on the stemloop structure of free singlestrand adenovirus DNA 25 What does the terminal protein do What is the function of nuclear factor 1
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