Continuum Models Biomed Engr
Continuum Models Biomed Engr BME 59500
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This 9 page Class Notes was uploaded by Brisa Lehner on Saturday September 19, 2015. The Class Notes belongs to BME 59500 at Purdue University taught by Staff in Fall. Since its upload, it has received 46 views. For similar materials see /class/207880/bme-59500-purdue-university in Biomedical Engineering at Purdue University.
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Date Created: 09/19/15
BME 5952 I CHM 5992 Fall 2005 Introduction to Biomedical Optics Lecture 13 Coherent AntiStokes Raman Scattering Microscopy 2 JiXin Cheng Exam 1 Average 84 2 Number 0 50 55 60 65 70 75 80 85 90 95100 Score CARS Arises from Third Order Susceptibility Crosspolarized Ep and ES Parallelpolarized Ep and ES 3 E E E 3 Z1221 P P s lellEPEPES 3 X1122ESEPEP 3 Z1212EPESEP Here 1 and 2 represent the polarization Kleinman s symmetry For an isotropic medium 3 3 3 3 Z1221 Z1212 Z1122 2511113 Third Order Susceptibility and the Polarizability Tensor EPolarizatsion vec zs ZS as I I I 25531 2a5z I I Z5221 2a 2 R 0 represents the polarized components 051 represents the depolarized components Probing Molecular Orientation Using Polarization Sensitivity of CARS Microscopy axx gtgt Uyy uxx gtgt 11 With xpolarized excitation ymmetry axis alongt e x direction give a strong CARS signal Lipid and Water Ordering in Multilamellar Liposomes Br y my my Cheng PauloLWeltZ anXme F NAS100 98262003 H20 Stretching Vibration Depolarization Ratio Raman depolarization ratio lt2 lt2 2 DR DR pie 0 12 O 11 751221 11111 75 for totally depolarized vibration modes 39 o es PR 0 0 ltpR lt 075 for symmetrIc vibration m d Kleinman s symmetry DNR pm ZSLNRJnm 13 Using PolarizationSensitive Detection for Background Suppression Find the Optimal Value of 1 to Maximize Vibrational Contrast X and Y components of the Polarlzatlon vectors nommsonant pan of PNR y Pf 323E E cosy Pf 311251193 sin Analyzer X and Y components of the resonant part of PR Rf 31511EIEE COS gt x RVR 3Z ZlEgEg Sin Oudar Smith Shen Appl Phys Lett 34 758 1979 PNR is linearly polarized with an angle of a from the x axis PNR 3szflEliE cos Moos 0 Where ais related to d by tenor pNR tany Pi is the projection of the resonant part along the analyzer Pi 3EEz n cosg sinai pk sin cosa Assuming extinction ratio of rfor PNR the vibrational contrast is PfPAiRrrZ 11ZZ 121pRpme2 Sinz 20 on Should be 45 thus the optimal value for is 716 Suppression of nonresonant background Polarization CARS spectra of liposomes p 135 q 45 Normalized CARS Int ZEUEI ZBUEI 3 El ZEUEI ZBUEI 3 El 4 mp 035 cm 4 wprwsl cm q Is the polarization angle of the analyzer Multiplex CARS MCARS gnilging and MicrOSPectr S Py 0quot the Same PolarizationSensitive Detection in MCARS e up Selective probing of the symmetric or asymmetric CHZ band 5 3 F 0 F 90 a 1 v E 391 us I u m 2mm 3mm ravensems1 2 2 m m MCARS using 1 Du synchronized plco Em 2m 3 E 2am mm mm mprms cm l mprms crrr1 and femtosecond beams 7 Cheng Volkmer Book Xle J Phys Chem B106 8493 2002 is D WO C 9 Is the polarization angle ofthe analyzer Summary of CARS Contrast Mechanisms Biophysical and Biomedical Applications Picopico excitation provides high sensitivity and high imaging speed Picofemto excitation permits spectral imaging with a compromise of sensitivity and speed Forwarddetected CARS is useful for probe large objects Molecular composition of Membrane Domains Epidetected CARS is sensitive to small objects and can g Myelin sheath and demyelinating diseases be used for in vivo imag n The polarization sensitivity of CARS can be used to probe molecular orientation and to remove the non resonant background In V39VO CARS Imag39ng CARS is a coherent imaging technique The CARS signal generation can be described by a Green s function formu a io In situ imaging of drug release Neurons Axons and Myelin Sheath Nodes ol Flanwe CARS Provides a Sensitive Noninvasive Probe of Myelin Sheath Spinal cord Myelin sheath contains 70 lipid and 30 protein CARS Imaging of Myelin Sheath in Its Natural State Acquisition time 39 11 seconds Resolution Lateral 07 pm Axial 03 pm lntenslty a u n u an m m an m m Pusmun m Pusmun w 1 Excitation Wang Fu Zlckmund Shh Cheng BOphys J 200589 5817591 Large Penetration Depth Into Spinal Tissues 4000 o o o o 2000 1000 Max CARS Int au 0 100 150 200 250 300 Depth Into Spinal Tissue um High 3D Spatial Resolution Node of Ranvier N SchmidtLanterman incisure a P V Bar5um Wang Fu Zlckmund 5m Cheng Bophys J 200589 5817591 Demyelinating Diseases HEALTHY N E RVE Multiple Sclerosis scarred myelin 391 myelin DAMAGED N E RVE Experimental Demyelination Model Lysolecithin or LPC H H OH P a 0 o O W 7N e H 0 EM image of LPG induced demyelination CARS images of Myelin Swelling Induced by LPC A Normal B Demyelinating Intensity a 15 Fu Wang Shi Cheng submitted for publication ACD2 vibration B CD2 vibration C peak dip eak 2080 cm391 2125 cm1 No I No No bilayer 7 39 bilayer I bilayer Probing 2x104 d62 DPPC molecules in the focal area 10pm Li Wang Cheng Biophys J 2005 in press n n F r Egan derec39a ed CARS Spec rm or Single Brimsaris DGNVMEI olecular Conczrri39m i39ion fricm Con39i39msi39 A d62DPPC bilayer 2 c 2125 cmquot B DOPC bilayer zmn 3 mquot quotquot Theoretical Model ACARS 2nAZr n is the number of vibrational oscillator in the focal volume n newppc 2n v 15 y0106x us Now ng 2min 2mm 215m 22mm 27mm 28mm 29mm 3mm r Wavenumbemmquot Wavenumberkmquot lt mquot u 2 40 an m mu 7 uezrDPPcvu Bila ers of DPPCd62DPPC at various molar ratios Li Wang Cheng Biophys J 2005 in press y aw B Tm Pm mm W 92W 7 Molecular Composition of Patch Domains quot 639 V quot quotQquot Characterized by CARS Confocal fluorescence images of Pi dark and LB red domains in GUVs of DOPCDPPC 28 A CH2 peakdip B ct2 peakdip A dark domain in a bilayer of DOPCdGZDPPC labeled with 311 c 267 c 249 ac 10pm BODIPY PC Red RhDPPE Green BODIPY PC Li Cheng submitted to PNAS Controlled Drug Release Stents coated with polymerdrug films Stents used in patients blood vessels for sustained drug release CARS Spectra of SIBS and PTX 28003100 cm391 Region 150018000m391 Region PTX PTX times 4 25 BS SIBS a o CARS Intensity au o CARS Intensity au o m W xx 1500 1550 1600 1650 who 173950 ia39oo Wavenumbercm391 0 2800 2900 3000 3100 Wavenumber cm391 Eunah Kang Kwon Haifeng Wang In situ CARS Imaging of PTX Release from SIBS Film SIBS 2840 cm391 250 SIBS 2840cm39l PTX1710cm39117C AJcm391 Intensity au Sample in 100 2propanol for 30 min In Vivo Nonlinear Optical Imaging In vitro model systems cultured cells Ex vivo live tissues In vivo live animals Inflammation and immune system response Clinical diagnosis EpiCARS Imaging of Peripheral Nervous System in vivo Parallel axons in sciatic nerve Fat Cells Brandon Huf f
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