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Lecture 6

by: AnnaClippinger

Lecture 6 BIOL-L211 2521

GPA 3.3
Molecular Biology
Megan Dunn

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About this Document

Lecture 6 notes and also notes on crossing over
Molecular Biology
Megan Dunn
Class Notes
25 ?




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This 10 page Class Notes was uploaded by AnnaClippinger on Monday September 28, 2015. The Class Notes belongs to BIOL-L211 2521 at Indiana University taught by Megan Dunn in Summer 2015. Since its upload, it has received 31 views. For similar materials see Molecular Biology in Biology at Indiana University.


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Date Created: 09/28/15
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remain together N MN Errand Parental strandlsibroki irij double stra ided fragmenisyused as 39 Du Q H a PM mmuw a X TH Wm Mre Me The MeselsnnStnhl Experiment m m gem nal Determine mechanism for DNA replication Overview H H mightiwrsian 0i Wino am e di itrniime Why nitrogen mmryoruremrw l j v H mimm mamarggses Allow bacteria to grow and replicate nu Wilda ramming swama lawn Sapa rate DNA by density whee phii W in NIWO gin imn V Boner1 0 we 0 ride new Grow bacteria in media containing one of two different isotopes of nitrogen heavy nucrmu grown in Ch F HM er Si 0 n3 0 Q5 Sam 1 l a V EMh 0 rm 4 wascommnmnmgen 1 ll Butlermi ails Will rareur nii m gen Ham rm media s Ulnar NA made winnqu are a 1 avi Di HQth Tmclg waqum 3a in hm MWN NH me Si ad D N A H 030mg The MeseisonStehl Experiment ll Goal Determine mechanism for DNA li39ep ca on 1 Grow bacteria in media containing nucleotide precursors labeled with 15M Hf i 0 39 Transfer to media containing nucleotide precursors labeled with 15M I H f V m R 1 sample 1 q Mimi on Cl ttr Hanger H H N gt i 3 Allow to grow in media with 14N l it m w eS we 1 E mlpw 7 QMQ39l atl mg gm u W t i hit ll QM DNA 39 El 0 MN Easieriagmwingmiam 21EEnamr V 39 this Which i r the i lMSi tats C Tlljtl39 0 H 0 thi thQ hltroqell PT39ESCW 5 m tint l5 umrlul DNA W the and Di Step 1 The MeselsonStahl Experiment 0 hr K l 1 Goal Determine mechanism for DNA replication l S OtopUGN 4 Place the three samples in an ultracentrifuge Pellet through a cesium chloride CsCl gradient D39NPx Si limdttd uxl oltm l l l hl er wthh ifo the haw st V DN H Wilma 0 me tel oi 4fquot g 1 g 1 me rubr e A 7 A 39f l I E AYhybrlderm 7 I gt I 1 All St paroled m mar OlQnJ lN a a r l The MeselsonStohl Experiment Goal Determine mechanism for DNA replication 5 Observe location of DNA molecules 141N314 light DNA 15N14N hybrid DNA i Ng l 5N heavy DNA before transfer to 391 4N 9 transfer to 14N Ht hr total 5 9K mt preclltt lim r c 5U its For 0 in S39Ewarwe 5 0 i s p ersrw m 0 bltl or mill tun on wartime Aim 1th l l ith lgn w gSN may I H Wbrld Mirt Clo lit i H ow PMquot two generations after transfer to 1 W one cell eneration after Conclusion DNR RQDHCMWR its S l m C m39il ll l l lfe WE 4 thin rm Welt DNA synthesis A LtualN 2 RtlJllcan on to r KS imam u in eukaryotes T Q rm 7 mm 7 mm Es thtli tint com a u n on Elite t 3m t qmarutton Di rtpncumm e D N it r am no on is NOT ConStrva xt 39 m equence speci c positions where roilieation is initiated 39 U til P l U l ll HS 0 i if Complex of proteins that melt the double helix O m L i I liigim Origin of replication h p tip it can on along 1m 0llquotl Both 1 M putN D Ni litquotint occu rim Bo m dl rwmw Parental etrantl Daughter strand l D W mgS prose Rt pH cation 5 Bobbitt Willqu lolqu f imam Contact Two daughter PINE molecules in future diagrams we will only o 139 11 wiijw39 J onsider one replication fork hitterffwwwaootesaseduf hcultyfsjaspetf Iiio212tl molgoula Ishtrnl mmmsxs huww w an e 39 U i rim Jmmw Xi39an The rep m on fork m 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