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by: Burdette Dooley


Burdette Dooley
GPA 3.84

Delana Nivens

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Delana Nivens
Class Notes
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This 2 page Class Notes was uploaded by Burdette Dooley on Saturday October 3, 2015. The Class Notes belongs to CHEM 3300 at Armstrong Atlantic State University taught by Delana Nivens in Fall. Since its upload, it has received 45 views. For similar materials see /class/217881/chem-3300-armstrong-atlantic-state-university in Chemistry at Armstrong Atlantic State University.

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Date Created: 10/03/15
Plant Bioremediation Examined By XRF and AA Some plants are known to uptake heavy metals from the soil This process is sometimes used as a method for bioremediation of soils In some cases the metal ions can be recovered from the soil and plants and processed in such a way that the metal can be recycled and used in other products You should do some background searching and reading on this topic for your report For this miniresearch project you will grow a set of plant seeds Half of your plants will serve as a control group and watered only with water The other half will be watered with a solution of a heavy metal ion You will need to start these plants growing the first week of the semester After the plants are established ie toward the end of the semester when you will start this laboratory analysis you will compare the concentration of metal ion in all 6 plant and soil samples by two instrumental techniques AA XRF The metals that you can choose from are Pb Cu Co Ni Zn Fe Only 23 groups can analyze for the same metal Planting and growing Follow the directions on the seed packet provided Grow at least 8 plants 4 controls and 4 metal ion plants this will allow you to have one data set that can be thrown out or just an extra plant incase one dies Plants will be growing under grow lights in the instrumental lab Be sure to label your plant containers Until the seeds germinate only water the plants with water After sprouting begin watering your metal ion plants 12 times per week with a l M solution of metal ion use the nitrate form and be sure to indicate the ion charge ie SnII or SnIV XRF Analysis Foliage qual quant Wash and pat dry a piece of leaf from each plant Perform a qualitativequantitative analysis of the plant material Have the instructor show you how to set up the instrument for mapping at least 5 positions on the leaf You need to make the balance of the material carbon Soil quantitative Prepare in potting soil a series of metal standards from 01 metal ion by weight to 1 metal ion by weight by mixing soil with the appropriate amount of solid metal salt Have the instructor show you how to collect the quantitative data on each soil standard as well as 6 unknowns one from each plant Graph the peak height versus concentration of metal ion Determine the selectivity coefficient for your metal via XRF analysis Use the calibration curve to determine the amount of metal in the samples Compare your amount calculated from the calibration curve to the amount determined by the instrument Do they agree Why or why not Foliage sample preparation for AA Weigh out approximately 25 of foliage cut up into small pieces with scissors and place in a beaker Dissolve the foliage sample carefully in the hood in 12 mL Nitric acid Then add 2 mL of sulfuric acid followed by 2 mL hydrogen peroxide Set a watch glass on top of the container Heat the sample with stirring until it boils and all foliage is dissolved This sample should then be vacuum filtered through a FINE clean glass fritted crucible A small amount of water should be used to rinse the crucible Note the volume of the sample in the filter ask try to aim for 2025 mL total volume Soil Sample Preparation for AA Dry your soil sample in a 100 C oven After cooling weigh out N5 g grams of soil and place a beaker Add 5 mL of l M ammonium acetate Stir the sample for 5 minutes Allow the soil to settle Decant the liquid and save it The extraction and decantation should be repeated 4 more times At the end you should use vacuum filtration to remove all the liquid from the soil sample Special notes Lead Add 5 mL of 01 M EDTA to each ask use 217 nm 07 nm slit Ni use the 35245 nm or 34l48nm line to avoid Fe interference 07 nm slit fuel lean Cu Add 5 mL of 01 M EDTA to each ask use 32475 nm 07 nm slit fuel rich Co use 24073 nm and a 03 nm slit Fe in soil add 5 mL of 01 M CaClz to each ask Add 1 mL of 5 8Hydroxyquinoline to each sample soil and leaf 24833 nm 07 nm slit Zn 213 8 nm 07 nm slits Method of Standard Addition follow method above for th e foliage samples Prepare a stock standard your metal ion using the AA standards provided Make the solution 100 mgL in l M HN03 Using 4 50 mL volumetric asks pipet the same known amount 25 mL of your sample into each ask reserve some incase you make a mistake you will have 4 asks for each of3 foliage and soil samples a total of 24 samples Treat the samples as follows To sample 1 add 2 mL of l M HN03 dilute to the mark with deO To sample 2 add 05 mL of metal ion standard 15 mL of l M HN03 Dilute to the mark To sample 3 add 1 mL of metal ion standard 10 mL of l M HNO3 Dilute to the mark To sample 4 add 2 mL of metal ion standard Dilute up to the mark Run each sample on the AA in triplicate according to the instructions provided and the wavelength given above Remember that this is a standard addition curve so you need to read either your Instrumental text or your quantitative analysis text to figure out how to


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