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by: Cordie Homenick III

BasicMicrobiology MIC201

Cordie Homenick III
CSU Pomona
GPA 3.91


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Class Notes
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This 1 page Class Notes was uploaded by Cordie Homenick III on Saturday October 3, 2015. The Class Notes belongs to MIC201 at California State Polytechnic University taught by WendyDixon in Fall. Since its upload, it has received 32 views. For similar materials see /class/218319/mic201-california-state-polytechnic-university in Microbiology at California State Polytechnic University.


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Date Created: 10/03/15
III INTRODUCTORY MICROBIOLOGY EXAM II 7 REVIEW INFORMATION Microbial nutrition a Know the 4 categories of nutritional types for bacteria Know why this leads to diversity in bacteria b Know photo or chemo depends on mechanism for producing energy and auto or hetero depends on type of carbon source used c Know the 4 groupings for nutrients Know some of the constituents of bacteria that require these nutrients d Understand the mechanisms by which nutrients enter a cell which require concentration gradient energy transmembrane proteins and how nutrients ow into or out of a cell e Know the details of the three mechanisms for active transport including where the energy requirement is involved in the process i ABC binding cassette secondary transporters group translocation f Know about different culturing methods and how they are used Understand why culturing is important and how culture methods can enrich for speci c bacteria Microbial growth a Know basics of bacterial cell division b Know about bacterial exponential growth and be able to perform calculations similar to the examples performed in class Know the two formulasiexponential growth formula based on doubling and the formula for calculating generation time c Know typical generation times for bacterial cells vs eukaryotic cells Normal growing bacteria and slow growing bacteria d Know the standard growth curve for bacteria and the 4 phases of growth e Know the methods for determining number of cells presented here Be able to determine dilutions and calculate orgml for PetroffHausser chamber and plate counts f Know factors that affect growth how they affect growth how they affect the growth curve and how bacteria are classified based on the conditions that allow for their growth Know what conditions are optimal for the majority of microbes and which conditions only some microbes can grow in Know a few of the microbes mentioned in class and the specific conditions where they grow best i pH gaseous environment temperature water activity salt concentration Know how water activity is defined Short Answer Questions 1 You will have a problem on determining the CFUml from the standard plate count method 2 You will have a question on one of the types of active transport for bacteria You will need to be able to describe the protein components involved in the process and how molecules are moved by proteins from outside the cell to inside the cell You also will need to indicate where energy is required and how it is being used in the transport system


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