Environmental Biology Lab
Environmental Biology Lab BIOL 1210
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This 5 page Class Notes was uploaded by Mervin Champlin on Monday October 12, 2015. The Class Notes belongs to BIOL 1210 at Georgia Southern University taught by Michelle Tremblay in Fall. Since its upload, it has received 34 views. For similar materials see /class/222024/biol-1210-georgia-southern-university in Biology at Georgia Southern University.
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Date Created: 10/12/15
Matt Hellmann Lisa Dlugolecki 7 Section C BIOL 1210 24 March 2010 Allelopathic Effect of Peppermint Menthepeperita 0n the Germination and Growth of Radish Seeds Raphanus sativus Abstract The experiment s purpose was to examine the allelopathic effect of peppermint oil on the growth and germination of the radish seeds The data we will collect will allow us to see how certain plants can affect one another in closeness because of their allelopathic effects In the experiment we introduced the radish seeds Raphanus sativus with peppermint oil Menthe peperz39ta and water and recorded the growth and germination of the seeds in the presence of either 5 or 10 micro liters of each liquid Both the graph and chart displayed inhibitory effects on the radish seeds while in the presence of the peppermint oil because more seeds germinated and grew larger while just under the presence of water After reViewing the data I can verify my hypothesis saying that peppermint oil has an inhibitory effect on the radish seeds was correct From the data I saw that crops such as radishes should be careful of what plants they are placed near because this experiment displayed the inhibitory effect that one plant s allelochemicals can have on another plant s growth and germination Introduction Allelopathy is a very important aspect of environmental biology in that allelopathy affects the growth and germination of plant species in the environment These affected plants can be important where they could help out in pollution and erosion prevention The experiment was created to examine the allelopathic effects of certain plant chemicals and how they can affect the germination and growth of a different species of a plant The growth was affected by peppermint oil Mentha peperz39ta and the germination was on radish seeds Raphanus sativus Wang et al 2010 also investigated the seed germination and seedling growth of cucumber C ucumis sativus I and mycekiak growth of F oxysporum when using the allelopathic effects of the stubble leachates 0 125 25 50 100 of soybean Glycine max wheat Triticum aestivum 1 and oat Avena sativa 1 It is hypothesized that the peppermint oil will have an inhibitory allelopathic effect on the growth and germination of the radish seeds and will result in overall less production from the growing seeds I obtained my information through other scientists work that have studied different allelopathic effects on plants Wang et al 2009 studied the inhibition of cucumber seed germination and the root exudates of wheat oat and soybean The scientist findings were that the wheat and soybean roots exudates at higher concentrations that promote the seed germination which is opposite of our hypothesis for the radish seed In the same source it says if the wheat and soybeans roots get to a low concentration then it will have an inhibitory allelopathic effect on the growth and germination of the cucumber seeds Another experiment by Meksawat et al 2010 examined the allelopathic activity of itchgrass Rottboellia cochinchinensis which can inhibit the seed germination and growth of weeds What the scientists found was that the allelochemicals from itchgrass can inhibit seed germination and plant grth better at closer distances The allelopathic activity of itchgrass in he soil can in uence the germination which can cause a reduction in the growth of the seedlings After examining the different results and effects of each experiment I came to my hypothesis that the peppermint oil will have an inhibitory effect on the radish seeds Materials and Methods The experiment in class s meaning was to understand the allelopathic effect of peppermint oil in referring to the growth and germination of radish seeds We used many specific details and steps in order for our experiment to be performed correctly Each group placed fiveradish seeds n each of two petri dishes that were provided for them Each petri dish gained a controlled amount of 3 ml of deionized water that was placed in the middle of each dish using a pipet Using the pipet we transferred 5 or 10 microliters of peppermint oil M entha peperz39ta into one petri dish and water into the other petri dish In the other 2 petri dishes we added the amount of peppermint oil and water that we did not use in the first petri dish The fifth petri dish will be used as a control According to the lab manual provided by Tremblay 2009 the petri dishes were then placed under uorescent lights at a temperature of 25 2 degrees Celsius for one week After the oneweek waiting period we then examined the petri dishes and measured the amount of seeds that had germinated and the length of each seedling including the root and shoot The data was then collected from the entire class and an average was calculated for each volume of substance that was added to the radish seeds Results After calculating the average seeds germinated and length for each substance and amount I created a table and chart to display the results Figure 1 Germination of Radish Seeds in Different Volume Amounts of Peppermint Oil and Water Avg Seeds Germinated 0 7 Peppermint Oil Peppermint Oil Water Water 0 ul 5 ul 10 ul 5 ul Sub stanceVolume Table 1 Average Amount of Seeds Germinated and Average Length Substance Volume ul Avg of Seeds Avg Length of seed mm Peppermint Oil 10 05 13 Peppermint Oil 5 3 2025 Water 10 2 325 Water 5 3 395 Table 1 shows the average seeds germinated for peppermint oil and water at 5 microliters and 10 microliters and it also shows the average length for peppermint oil and water To better display the results for the amount of seeds germinated I created a graph to show the data and its correlation Discussion After analyzing the data and results from the experiment that we performed in class it is evident that peppermint does have an inhibitory allelopathic effect on the radish seeds This is evident in Figure l the graph shows that the seeds treated exclusively with water germinated much more frequently than those treated with peppermint oil As can be seen in Table l the seeds treated with water also grew larger in terms of length This proves that the hypothesis that peppermint oil does have an allelopathic effect on the growth and germination of radish seeds These findings can be important because they can show how chemicals such as peppermint oil can affect plants like radishes The data in the end supports my hypothesis because peppermint oil did have an inhibitory effect on the growth and germination of the radish seeds From this data we can
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