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by: Ashlee Wilson

Microbioloy Biol 1230

Ashlee Wilson
University of Memphis
GPA 3.3

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Chapter 6 Notes
King-Thom Chung
Class Notes
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This 3 page Class Notes was uploaded by Ashlee Wilson on Tuesday March 22, 2016. The Class Notes belongs to Biol 1230 at University of Memphis taught by King-Thom Chung in Spring 2016. Since its upload, it has received 12 views. For similar materials see Microbiology in Biology at University of Memphis.

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Date Created: 03/22/16
Microbiology Chapter 6 Growth and Culturing of Bacteria Growth and Cell Division  Growth  Binary Fission  Budding Bacterial Growth Curve  Lag phase  Log phase (exponential rate)  Stationary phase  Death phase (decline phase) Measuring Bacterial Growth  Serial dilution/ Standard plate counts  Counting colonies  Direct microscopic counts  Filtration  Turbidity  Colorimeter  calibration Direct Microscopic Count  Peteroff--Hausser Counting Chamber  Living and dead not distinguishable  Direct count is not viable count,  and can only count bacterial number  reaches 2 x 10 per /ml or above. Physical Factors Affecting Bacterial Growth  Oxygen  Moisture  Hydrostatic pressure Radiation  U.V. and Gamma Rays  Protective Mechanisms  Pigments  Enzyme repairing systems  Radiation resistant:  Deinococcus radiodurans Nutritional Factors Affecting Bacterial Growth  Fastidious microorganisms: special nutritional needs that can be difficult to meet in the laboratory.  Viable but not culturable microorganisms.  Carbon source:  CO, CO ,2or organic Carbon sources  Nitrogen source:  NO3, NH4, amino acids, peptons, proteins, or other nitrogen- containing compounds  Sulfur/ phosphorus  H S, SO , PO ---. 2 4 4  Trace elements; Tiny amounts of minerals such as copper, iron, zinc, cobalt, cofactors, K, Na, Ca, Mg, Mn, in enzymatic reactions.  Vitamins- coenzymes, folic acid, Vitamin K, B, B12, etc.  Nutritional complexity Locations of Enzymes  Extracellular: Exoenzymes, Table 6.2, P162  Periplasmic  Cytoplasmic  Adaptation of limited nutrients Sporulation  Endospores Other Spore-like Bacterial Structures  Cysts: Azotobacter- lack dipicolinic acid, not a means of reproduction  Conidia: Like fungi  Micromonospora,  Streptomyces  asexual spore, for reproduction. Culturing Bacteria- Streak Plates  Streak Plate Method  Pour Plate Method Culture Media  Types:  Synthetic medium -Defined synthetic medium  Complex - Chemically nondefined medium, blood, beef extract, yeast extract, peptone, soybeans, etc.  Commonly used media - peptone from meat or fish in nutrient birth, solid agar -yeast extract, casein hydrolysate, milk protein, serum etc. Culture Media Purposes  Selective media.  Differential media- SPS agar, MacConey agar  Enrichment media Culturing Conditions--- O2  Controlling oxygen content  Candle: use up oxygen, and provide CO 2  CO i2cubator  Maintaining cultures- stock culture, aseptic techniques  Preserved Cultures- Lyopilization (freeze-Drying), Reference culture: preserved culture that maintained the organisms with original characteristics of all known species and strains of bacteria. ATCC.  Diagnostic Tests- the enterotube multitest system, the Analytical Profile Index (API)  Limitations


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