BIOL LAB SCI MAJ I
BIOL LAB SCI MAJ I BIOL 1208
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This 31 page Class Notes was uploaded by Charles Kohler on Tuesday October 13, 2015. The Class Notes belongs to BIOL 1208 at Louisiana State University taught by E. Wischusen in Fall. Since its upload, it has received 67 views. For similar materials see /class/222811/biol-1208-louisiana-state-university in Biological Sciences at Louisiana State University.
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Date Created: 10/13/15
Biology 1208 Writing Assignment 2 Results Worksheet Jordan Conner Garrett Hamilton C02 Production If one adjusts the intensity of light on the plant then the consumption of carbon dioxide will not be affected is the null hypothesis for this experiment containing carbon dioxide and LED light Contrariwise if one does revise the light intensity in each trial then a difference in the consumption of carbon dioxide will result as the effect of this reaction In other words the amount of light on the plant will affect the amount of carbon dioxide consumed The micromoles of carbon dioxide consumed per second per moles squared decreases as the intensity of the LED light rises The strength of the light does in fact affect the amount of carbon dioxide the plant consumed alternative hypotheses When 01471449 micromoles of carbon dioxide were consumed the LED light did not affect the plant s consumption of carbon dioxide when the light was intensified from 800 to 1000 uMmzs Overall as the light was strengthened the consumption of carbon dioxide decreased 025 t I O 8 0 2 if 39 6 Q Q quot1 0 15 9 Q E E o 0 1 U 8quot 005 O V Q g 0 1 0 200 400 600 800 1000 1200 LED Light Setting uEmzs Figure l The micromoles of carbon dioxide consumed per second per moles squared as the LED light setting is increased by 200 uEmzs Based on the outcomes of the experiment the null hypothesis was not supported from the fact that the strength of the light had a factor in carbon dioxide consumption The consumption results were determined upon the strength of the LED light setting The alternative hypothesis was supported by the various consumption amounts that were determined based on the different levels of light because the null hypothesis was not supported The amount of carbon dioxide produced is related to the level of LED light setting on the plant In Class Quiz 12 mins Only REVISION Techniques Pipetting Spectrophotometry ENZYMES Biocatalysts Highly specific Most are proteins by nature Main function increase rate of a reaction by lowering activation energy NEW TERMS Rate of a Reaction A B gt C Activation energy Lighting of a candle burning of a matchstick Relative Energy A typicol energy profile Activated complex Uncatalyzed reaction E0 Energy of Activation Activated complex Catalyzed reaction Reactants Products Progress of Reaction Enzyme Substrate complex Lock and Key model Induced Fit model H21ydenMcNeil Publishing Inc Enzyme has a complex 3D shape conformation Enzyme starts with an empty Active Site catalytic center Substrate enters active site enzymesubstrate complex Induced fit allows the reaction to proceed Enzyme releases the product amp emerges unchanged from the reaction Enzyme may now bind to another substrate REVISION Enzymes video Exercise 1 Enzymatic oxidation of Catechol Catechol Oxygen Benzoquinone W OH o 0 my Mi E azl nm m Enzyme catechol oxidase How are we going to measure rate of the reaction Catechol Oxygen gt Bwater CO Observe the rate of conversion of catechol colorless to benzoquinone yellowish brown with time Rate Conc Of Benzoquinone product Time Exercise 1 Oxidation of Catechol to Benzoquinone OH Enzyme Source Potato Extra ct OH O Catechol Ox1dase 12 02 gt H20 Catechol Benzoquinone Colorless Yellow gt Brownish Blank amp Sample Mixed the contents of both tubes 1 mL Enzyme gt lt 1 mL Enzyme lt 5 mL Cafechol 8 mL dHZO 3 mL dHZO Blank Sample WARNING WHEN THE ENZYME amp SUBSTRATE ARE TOGETHER THE REACTION HAS BEGUN DON T CROSS CONTAMINATE PIPETS Procedure Add distill water in both the tubes Then add catechol to the sample tube and wait Set the wavelength to 500 nm and set Transmittance to 0 Add potato extract to blank tube mix well and measure Blank Insert blank and set Transmittance to 100 Then add potato extract to sample tube mix well switch Mode to Abs and measure sample Writing Assignment 1 CLEAN AREA FIRST Submit Graph NCR of Observation table Time secs Absorbance Conc mM ODU O 30 6O 90 120 150 180 What did you learn How did the catechol oxidase affect the rate of the reaction How does the absorbance value odu of the benzoquinone product relate to its concentration Calculating Gone and plotting it We time Convert Absorbance values odu to Concentration mM using the Standard Curve provided Plot on graph Making graphs 1 Choose the type of graph that best represents the data Line graph Leaf Ienght mm l 2 3 4 Collection number day Monkeygrass Turf When the one variable dependent represents a continuous function of the other independent variable eg Daily increase in leaf length between two different types of HFQCCDC Bar graph Proponion of has When the independent variable presents qualitative categories eg Proportion of reproductive and nonreproductive bats visiting two different types of habitat Graph Details 2 Put information on 3 Label both axes appropriate axis and include x Independent variable what you manipulated am y Dependent mater variable what you measured y X Time Fears Graph Details 4 Use easy to read and even increments on both axes 5 Plot the points use em 15 EXCEL 6 Do NOT connect the dots use a bestfit or trendline Bestfit line Planning for Enzymes Exercise 2 Exercise 2 An enzyme s shape is central to its function The 3D Shape is affected by the environment How do the different factors listed below affect catechol oxidase activity Enzyme concentration pH Substrate concentration Temperature Structure of the substrate Source of the enzyme Scientific Method Observations Te Model or generalization Predictive Statements Hypotheses Hypothesis HO null hypothesis no difference between the control and the treatment group Ha alternative hypothesis there will be a difference between the control and the treatments N ull Alternative Examples Study Area quiet noisy Low calorie green healthy food Use of filters in water treatment Hypothesis vs Prediction Hypotheses will only state whether we will or will not see a difference between 2 groups A PREDICTION states in what direction we will see that difference Predictions are more specific than hypotheses and more interesting EXAMPLE FertilizerTomato Plant Experiment Interested in testing whether fertilizer will have an effect on the number of fruits produced by a tomato plant Null Hypothesis There will be no difference in the number of fruits produced by the plant grown with the fertilizer compared to the plant grown without the fertilizer Alternative Hypothesis There will be a difference in the number of fruits produced by the plant grown with the fertilizer compared to the plant grown without the fertilizer Prediction The plants grown with the fertilizer will prod more fruit than the plants grown without fertilizer Analyzing enzymes and its Kinetics The enzymatic oxidation of catechol WA 1 due in class 30th Jan Oxidation of Catechol lnvestigative Exercise WA 2 based on this lab Feb6m Data Analysis amp Writing Presentations Feb 13th Next class Complete the prelab quizzes clue You can complete the NCR sheets and submit them today or give it to me next class Remember to bring the fruitvegetable all those who are doing the source of the enzyme experiment
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