COMP MTHDS ECSE 6963
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This 11 page Class Notes was uploaded by Miss Damien Crooks on Monday October 19, 2015. The Class Notes belongs to ECSE 6963 at Rensselaer Polytechnic Institute taught by Badrinath Roysam in Fall. Since its upload, it has received 34 views. For similar materials see /class/224771/ecse-6963-rensselaer-polytechnic-institute in ELECTRICAL AND COMPUTER ENGINEERING at Rensselaer Polytechnic Institute.
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Date Created: 10/19/15
ECSE6963 BMED 6965 Cell amp Tissue Image Analysis Lecture 2 Molecular Bioimaging Part I Badri Roysam Rensselaer Polytechnic Institute Troy New York 12180 a Re W a y a 11586 361quot w hhhhhhhhhhhhhhhhhh a Center for SubSurface Sensing amp Imaging Systems Recap Image Analysis is the business of making quantitative measurements from images We focus on images of biological cells tissues and organisms captured by modern optical microscopes 0 Today We ll review some biology basics to establish a basic vocabulary that we can use We ll introduce some imaging fundamentals Recall Cheek Cell Under a Micro cytoplasm plasma membrane nucleus Cell Under an Electron Micrscope an 2 j Much greater detail But no molecular selectivity Can t do live cells csc images quot 739 7 Molecular Imaging of Cells 0 Molecular Image a spatial map of how a selected molecule is distributed To do molecular imaging we need a way to sense only the molecules of interest while ignoring all other molecules in the cell For this we need to understand some basics of molecules and how they interact with photons How Light and Molecules Interact Excited State quotv or um r jig l 7 U LC ll mwmhma Q om 511 Every molecule has a characteristic set of energy levels Energy levels correspond to speci c states of how electrons are distributed molecular rotation vibration bending etc Adding energy can make it go to a higher energy level and vice versa Important fact Molecules are very selective Transitions between some states are allowed and others are forbidden due to quantum mechanical constraints The set of states and transition probabilities define a molecule s optically observable signature Light Absorption E 2 Absorption can only occur when hv E photon M I hV E2 E1 E 1 If the energy of the probing photon is mismatched to the energy difference between the quantum states the molecule does not absorb the photon and is effectively transparent at the frequency v Absorption is fast z 103915sec Absorption is proportional to concentration of molecule and path length but independent of intensity of excitation The Absorption Spectrum Absorption L Frequency V The absorption spectrum of a molecule is the absorbance as a function of wavelength sometimes this is a su icient molecular signature by itself although more commonly it is only a part of the overall molecular signature Imaging Spectroscopy Multispectral Imaging Put a wavelength selective a evice eg a filter prism or a a i raction grating in front of the image sensor to collect images at multiple selected frequencies Each pixel has N values one for each frequency NH Lumen Tumor Ce Blood VSSSBI I 550 600 550 700 750 I 500 Wavelength iquot quotm What happens after a molecule has absorbed a photon Choice 1 Photobleaching Molecule gets damaged irreversibly We try to avoidminimize this Choice 2 Heat dissipation Not very interesting from an imaging standpoint Choice 3 Emission of a photon Very interesting from an imaging standpoint we can detect it
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