New User Special Price Expires in

Let's log you in.

Sign in with Facebook


Don't have a StudySoup account? Create one here!


Create a StudySoup account

Be part of our community, it's free to join!

Sign up with Facebook


Create your account
By creating an account you agree to StudySoup's terms and conditions and privacy policy

Already have a StudySoup account? Login here


by: Ashley Kunze
Ashley Kunze
GPA 3.61

N. Reich

Almost Ready


These notes were just uploaded, and will be ready to view shortly.

Purchase these notes here, or revisit this page.

Either way, we'll remind you when they're ready :)

Preview These Notes for FREE

Get a free preview of these Notes, just enter your email below.

Unlock Preview
Unlock Preview

Preview these materials now for free

Why put in your email? Get access to more of this material and other relevant free materials for your school

View Preview

About this Document

N. Reich
Class Notes
25 ?




Popular in Course

Popular in Chemistry and Biochemistry

This 3 page Class Notes was uploaded by Ashley Kunze on Thursday October 22, 2015. The Class Notes belongs to CHEM 142B at University of California Santa Barbara taught by N. Reich in Fall. Since its upload, it has received 85 views. For similar materials see /class/226944/chem-142b-university-of-california-santa-barbara in Chemistry and Biochemistry at University of California Santa Barbara.

Similar to CHEM 142B at UCSB

Popular in Chemistry and Biochemistry




Report this Material


What is Karma?


Karma is the currency of StudySoup.

You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!

Date Created: 10/22/15
Learning goals LG and guidelines G for studying each chapter Your reading for each chapter should be guided by two considerations First look at the number of days we will spend on a chapter typically 23 days Divide the chapter pages into the corresponding sections halves thirds etc Second the online quizzes in class quizzes and iClicker questions will follow this same approach so you need to read the appropriate sections A proven strategy to cover this amount of material is to do as many of the problems as possible The only way you will be prepared for the quizzes and iClicker questions is to both read and practice problems Chapter 6 Learning Goals After completing Chapter 6 you should be able to l gt t O 00 ONUIAUJ N b Il ID I llbl ON Design an experiment which can falsify an alternative explanation to what you are proposing Identify and describe coenzymes and cofactors and explain how they can help enzyme reactions Be able to classify an enzyme on the basis of the reaction it carries out Describe the rate enhancements typically displayed by enzymes Explain how the active site of an enzyme is important to the Michaelis complex Identify those features in the energy landscape of an enzymecatalyzed reaction which are altered by the enzyme and those which are not Identify the relative contributions of intermolecular forces towards enzyme catalysis and how these change between the ES and E81 Explain how binding energy leads to rate enhancements and specificity in enzyme catalyzed reactions To identify when acidbase covalent and metal ion assisted catalysis occur in enzyme catalyzed reactions To identify when covalent intermediates occur in enzyme reactions and how such intermediates lead to rate enhancements Use enzyme kinetics to understand mechanism Use km to determine enzyme concentration and use km to determine how long it takes for an enzyme to convert substrate to product Determine when the production of ES and its breakdown is balanced Determine the condition when Etotal ES To estimate Km from a velocity vs substrate concentration plot and do so precisely using the MichaelisMenten equation Explain that Km Kd is not necessarily true and under what conditions and how Km and Vm can mean different things for different enzymes 1 am not referring to different values Determine which of two substrates given their Vmax and Km values are preferred by an enzyme Propose using product inhibition data what the kinetic mechanism of atwo substrate reaction is Learning goals LG and guidelines G for studying each chapter Your reading for each chapter should be guided by two considerations First look at the number of days we will spend on a chapter typically 23 days Divide the chapter pages into the corresponding sections halves thirds etc Second the online quizzes in class quizzes and iClicker questions will follow this same approach so you need to read the appropriate sections A proven strategy to cover this amount of material is to do as many of the problems as possible The only way you will be prepared for the quizzes and iClicker questions is to both read and practice problems Chapter 12 LG upon nishing this chapter students will be able to lExplain how signaling molecules eg thyrotropin releasing hormone often work on only one subset of cells and the factors that contribute to this being so sensitive 2 Why does signaling often occur with a geometric increase rather than linearly 3 Devise an experimental protocol to determine the affinity of a receptor for its ligand and the number of receptor molecules in a sample 4 Explain how the modularity of signaling pathways contributes to their being so conserved evolutionarily 5 Explain the molecular distinctions between the six basic receptor types G protein coupled tyrosine kinase guanylyl cyclase gated ion channels adhesion rececptors and nuclear receptors 6 Explain why the plasma membrane receptor is essential for the GPCR pathway 7 Explain how agonists and antagonists can be so structurally similar with a specific focus on the adrenergic receptors and epinephrine 8 Explain how second messengers like cyclic AMP serve to convert primary signals into downstream events how cyclic nucleotide phosphodiesterase contributes to controlling the levels of cAMP 9 Explain how the lifetime of the GTP is controlled and how this control contributes to the GTPase switch 10 explain how cholera and whooping cough toxins lead to the overproduction of cAMP ll Explain how one molecule of epinephrine can lead to the release of 100000 molecules of glucose into the blood 12 Explain how other second messengers diacylglycerol inositol triphosphate Ca2 are alternative examples to cAMP both in structure and in mechanism 13 Predict the effect of using BFP and mRFPl GFP variants figure 4Box 123 in a FRET experiment 14 Compare and contrast the role of Ca2 as a secondary messenger and its reliance on calmodulin with the IP3 pathway 15 Describe how some receptors can activate adenylyl cyclase whereas others inhibit it leading to cellular cAMP levels being the integrated input from two or more signals 16 Explain how the autophosphorylation of receptor tyrosine kinases allows it to modify other proteins thereby ensuring the signaling cascade l7 Propose how the SH2 domain leads to the Ras signal transduction 18 Explain how hormone signals lead to protein phosphorylation by PKA which leads to phosphorylation of additional kinases as in the insulin and MAPK cascades 19 Compare and contrast the two major routes of signaling direct effects by phosphorylation and indirect proteinprotein mediated by protein kinases 20 Explain the importance of membrane potentials to ion channels in various cell types including neurons 21 Explain the importance of integrins to cellcell recognition as well as for transmitting signals between cells To predict the loss of regulated adhesiveness to the homing of leukocytes to an infection 22 To compare and contrast the mechanism of action of nuclear hormones like retinoic acid and thyroid hormone with other hormones like insulin 23 To explain the importance of the response regulator in the two component bacterial signaling pathway in allowing cells to move towards a nutrient gradient 24 To compare and contrast the bacterial two component and plant two component detection pathways eg ethylene detection describe the similarities differences 25 Predict the consequence of causing cAMP levels to be signi cantly lowered on the vision sensory system 26 Predict the effect of vitamin A deficiency on your retinal levels 27 Explain the importance of GPCRs to vision olfaction and gustation 28 Predict and explain the impact on vision of using a nonselective phosphodiesterase inhibitor 29 Explain the importance of cyclindependent protein kinases on the cell cycle 30 Compare and contrast the different mechanisms to regulate the cyclinCDK complex during the cell cycle eg CDK synthesis cyclin degradation dephosphorylation 3 1 Explain the origins of oncogenes 32 Explain the role of the GTPase activity of the ras oncogene in its tumorigenic activity 33 Explain the remarkable specificity of the protein kinase inhibitor Gleevec used to treat certain forms of leukemia Guidelines We will spend three days on this chapter divided into approximately pages 433460 460473 and 473494 Rather than memorizing Figure 121 learn what each of these processes entails how they differ the associated problems end of chapter guidebook etc can help emphasize this ditto for all the following sections advice Box l2l describes a widespread and important approach used throughout biochemistry and drug studies Scatchard know it well Figure 122 nicely summarizes the different types of signal transducers described in detail in the chapter know all of them well The beta adrenergic system is iconic know it well the structures and narrative e g Figure 123 figure 124 are important Know the protein kinase system GTPase switch and side bar story Figures 125 6 and Box 122 The cascade described in Figure 127 is extremely common know how it works because it comes up repeatedly throughout the quarter How are beta adrenergic signals reversed see Figure 128 and related text problems Don t memorize Table 123 rather know what cAMP is name structure and that it functions in diverse pathways of HUGE biological importance What does figure 129 tell you about how kinases are regulated work together where they are located etc DON T memorize Table 124 rather know what phospholipase isdoes what the name structure of IP3 is and how they work together with divalent calcium see Figure 1210 Box l23 describes Nobel prize winning science know it the structuresmechanism figure 3 the spectral region Figure 4 not the


Buy Material

Are you sure you want to buy this material for

25 Karma

Buy Material

BOOM! Enjoy Your Free Notes!

We've added these Notes to your profile, click here to view them now.


You're already Subscribed!

Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'

Why people love StudySoup

Jim McGreen Ohio University

"Knowing I can count on the Elite Notetaker in my class allows me to focus on what the professor is saying instead of just scribbling notes the whole time and falling behind."

Janice Dongeun University of Washington

"I used the money I made selling my notes & study guides to pay for spring break in Olympia, Washington...which was Sweet!"

Steve Martinelli UC Los Angeles

"There's no way I would have passed my Organic Chemistry class this semester without the notes and study guides I got from StudySoup."

Parker Thompson 500 Startups

"It's a great way for students to improve their educational experience and it seemed like a product that everybody wants, so all the people participating are winning."

Become an Elite Notetaker and start selling your notes online!

Refund Policy


All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email


StudySoup has more than 1 million course-specific study resources to help students study smarter. If you’re having trouble finding what you’re looking for, our customer support team can help you find what you need! Feel free to contact them here:

Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to

Satisfaction Guarantee: If you’re not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.

Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.