PLANT MOLECULAR BIOLOGY
PLANT MOLECULAR BIOLOGY PLS 620
Popular in Course
Popular in Soil Science
This 6 page Class Notes was uploaded by Joseph Collins on Friday October 23, 2015. The Class Notes belongs to PLS 620 at University of Kentucky taught by Arthur Hunt in Fall. Since its upload, it has received 21 views. For similar materials see /class/228283/pls-620-university-of-kentucky in Soil Science at University of Kentucky.
Reviews for PLANT MOLECULAR BIOLOGY
Report this Material
What is Karma?
Karma is the currency of StudySoup.
You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!
Date Created: 10/23/15
Tobamoviruses type member tobacco mosaic virus single stranded RNA viruses genomic RNA is messagesense RNA strand linear genome of about 60006500 nt genomic RNA is capped and has a nonpolyadenylated 339 end 339 end of genomic RNA can assume a tRNAlike structure can be aminoacylated by tRNA charging enzymes and modified by CCA adding enzymes genomic RNA encodes 34 open reading frames all are encoded by the same RNA strand RNA genome encodes just 34 quottranscriptsquot or subgenomic RNAs 9 how are the possible open reading frames expressed if they are how does the RNA genome replicate how does the virus move from cell to cell from infected to uninfected plant 39I 20 7 s2 OAS 126K 183K54K 30K 17K 39T 5 3 H 5 i RIds 3 5 3 539 3 539 339 subgenomic RNAS Pr igjny CP RNAs 30K 126K183K replication 126K 183K protelns 30K CP Functions of the open reading frames 126183 kD protein probable virusencoded component of the replicase RNAdependent RNA polymerase mutants are not viable sequence shows significant homology with other known RNAdependent RNA polymerases 126 kD protein has capping activity 30 kD protein cellcell movement protein shortdistance movement purified protein binds nonspecifically to single stranded nucleic acids mutants do not move from cell to cell ts mutants have been identified and characterized cloned wt 30 kD protein can complement movement mutants protein is localized to plasmadesmata protein can change increase plasmadesmatal exclusion limit coat protein sole protein component of the virus a determinant of resistance mutants affect hypersensitive response and long distance movement mm three quotmRNAsquot and thus three quotpromotersquot promoters are recognized by the viral replicase and mRNA synthesis is analogous to genome replication no dsDNA intermediates genomic mRNA has 539 and 339 translational enhancers 539 enhancer is a probable highaffinity internal ribosome binding site 339 enhancer is functionally analogous to polyA tails works in conjunction with the 539 enhancer not with 539 cap structures different subgenomic mRNAs are expressed at different times in the infection process seems to be determined by the quotpromoterquot 3 lLBN 1 two step synthesis of a genomelength strand intermediate then synthesis of genomic and subgenomic RNAs 339tRNA structure is important for replication quottelomeresquot host proteins are probably involved CP RNAs 30K 126K183K Q OAS replication 126K 183K54K 30K 17K 126K 183K protelns 30K CP 5 i 3 H 5 RI ds 3 5 3 5 3 539 339 subgenomic RNAS Pr igjny CF RNAs 6 30K 126K183K Q OAS replication Wildrtype 126K 183K54K 30K 17K 126K 183K protelns YFG RNAS 30K 126K183K replication 2 OAS gene replacement 126K 183K54K I 30K IYFG 126K 183K protelns 30K W YFP YFP CP RNAs 30K 126K183K OAS replication 9 5 gene addition I 126K 183K54K 30K 17KIY Fl 126K 183K 30K protelns CP YFP
Are you sure you want to buy this material for
You're already Subscribed!
Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'