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Physiologcl Psyc Honors

by: Tremayne Beer

Physiologcl Psyc Honors PSYC 3306

Marketplace > University of Memphis > Psychlogy > PSYC 3306 > Physiologcl Psyc Honors
Tremayne Beer
University of Memphis
GPA 3.84

Charles Long

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Charles Long
Class Notes
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This 4 page Class Notes was uploaded by Tremayne Beer on Friday October 23, 2015. The Class Notes belongs to PSYC 3306 at University of Memphis taught by Charles Long in Fall. Since its upload, it has received 16 views. For similar materials see /class/228447/psyc-3306-university-of-memphis in Psychlogy at University of Memphis.

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Date Created: 10/23/15
Physiological Psychology Charles J Long PhD TECH NIQU ES This course outline is designed to provide you with a study guide Use the lecture book and other resources to expand on its contents OUTLINE TOPICS I Objectives I Histology l Tracing l Neurochemical l Radiological l Ablation I Recording TERMS QUIZ l RETURN Learning Objectives Describe the steps in tissue preparation and discuss the importance of each Discuss the basic differences between a cell body and a myelin stain Explain how neural connections are traced Discuss how autoradiography is used to measure metabolic activity Discuss the similarities and differences between CT lVlRl PET and SPECT scans Describe ablation procedures and explain the advantages and disadvantages of each Explain the differences between EEG and evoked potential recording OOlOUiIgtUJN Discuss the important limitations of ablation and recording in determining brainbehavior relationships 0 Discuss reversible lesions and describe the advantages and disadvantages as compared with RF lesions Histological Procedures Tissue Preparing Techniques 0 Perfusion o Fixation Destroy autolytic enzymes 0 Sectioning o Microtome 1080 um thick http14 l 22514185ugpnpcssOOthml 1 of 6 2192001 61223 PM Physiological Psychology 9 Techniques 0 freezing quick 0 embedding paraffin sectioning 0 Mounting o albumin 0 Staining 0 Cell Body Nissl substance 0 RNA and DNA 0 Cresyl Violet most common 0 Myelin Stains axons o Membrane Stains O GolgiCox stain silver 0 Autoradiography O Inject radioactive substance 0 Labeled amino acids taken up by cell 0 Brain sections coated with sensitive emulsion develop like photograph Tracing Neural Connections Axoplastic Transport transport of material along neurotubles Tracing Efferent Axons o Anterograde labeling use radioactive amino acit then autoradiography to monitor ow down axon along neurotubles o Lectins stain cells of immune system 0 Immunocytochemical methods take advantage of immune reaction Tracing Afferent Axons o Retrograde labeling methods taken up by terminal endings amp carried to cell body 0 Horseradish perioxidase Chemical injected into region 0 Taken up by terminal boutons o Transported to cell body 0 example Flurogold Horseradish peroxidase 0 Enzyme o Picked up at ends of neuron amp transported back to cell Tracing Brain Connections http14122514185ugpnpcssOOthml 2 of 6 2192001 61223 PM Physiological Psychology 9 Techniques 0 After person dies the brain is fixed 0 Dil crystals placed on fiber tract 0 Travel by diffusion to ends of fibers 0 Under UV light it appears flourescent Neurochemical Procedures Three basic ways of locating of neurons that produce chemicals 0 Localizing the chemicals 0 Localizing the enzymes that produce 0 Localizing the messenger RNA for synthesis Peptides are localized directly Auto radiography Measuring Metabolic Activity Radioactive 2deoxyglucose 0 Form of glucose taken up by cell 0 High rate activity takes up most Genetic activity 0 Neuronal activity genes turned on O Fos protein staining Neuroradiological Procedures Brain Imaging Structural Measures 0 Skull Xray o Cerebral angiography dye in vessels amp Xray o Pneumoencephalograph air in ventricles amp Xray Computerized Axial Tomography CT Scan 0 Xray emitter amp detector 0 Brain scanned from all angles circular Scannin 1 we http14122514185ugpnpcssOOthml 3 of 6 2192001 61223 PM Physiological Psychology 9 Techniques Magnetic Resonance Imaging MRI 0 Doesn t use Xrays 0 Strong magnetic eld 0 Nuclei emit radio waves 0 Detects radiation from H molecules Functional Imaging Regional Cerebral Blood Flow 0 Inhale radioactive isotope amp use sensors on scalp to detect distribution Positron Emission Tomography PET o Inject radioactive 2DG o Decay of radioactive isotopes emit positrons o Detected by PET scanner 0 Requires onsite cyclotron Single Photon Emission Computerized Tomography SPECT Scan 0 Less expensive but less spatial resolution Functional Magnetic Resonance Imaging fMRI 0 MRI sensitive to chemical environment of neural tissue 0 Active neurons increased blood flow 0 Doesn t require isotope injection 0 Higher resolution than PET WI Problems with Functional Imaging 0 Time course of events that these methods measure is slow min but neural events fast ms 0 Measures metabolism not cognitive functioning Ablation 0 Doesn39t tell function rather What rest of brain can do in its absence 0 Brain areas highly interconnected o Nuclei vs fibers of passage 0 Bilateral organization 0 Aspiration 0 Electrical Lesions http14 l 22514185ugpnpcssOOthml 4 of 6 2192001 61223 PM


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