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Cell Biology Lab

by: Amy Lehner

Cell Biology Lab BIOL 3111L

Amy Lehner
GPA 3.85


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Class Notes
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This 11 page Class Notes was uploaded by Amy Lehner on Sunday October 25, 2015. The Class Notes belongs to BIOL 3111L at University of North Carolina - Charlotte taught by Staff in Fall. Since its upload, it has received 18 views. For similar materials see /class/228982/biol-3111l-university-of-north-carolina-charlotte in Biology at University of North Carolina - Charlotte.


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Date Created: 10/25/15
Lecture I 5 Influenza virus PART 2 replication transcription CLASS V ss RNA viruses Genome Replication RdRp RNA synthesis RdRp ss RNA genome a 5 1 RdRp 5 39 39 7 3 t RdRp 3 ss RNA genome 5 l RdRp lt Viral mRNAs gt 5 3 51TZZf 552213 1 NO DNA Against Central Dogma 2 Genomic RNA is complimentary to mRNA cannot be directly translated 3 Virions must carry their RdRp from cell to cell mRNA positivestrand or positivesense RNA or RNA Laboratory Diagnosis Officebased rapid tests ELISA assays Some can distinguish between Influenza r A and B Fast results 1 to 20 minutes Some falsepositive or negative results f Other TGStS Figure 12cso1An influenza Cell Culture inoculate MDCK cells teSt39 most accurate test Serology Analyze convalescent serum and analyze for an increase in antibody titer 1 I I 1 Courtesy and nnnnnnnnnnnnnnnnnnnnnn my Cellular Pathogenesis To mouth Droplet transmission Virus enters respiratory tract Attaches to ciliated columnar epithelial cells lining the sinuses and ainNays particle Mucus l a To lungs Figure 123a The cilia are attached to columnar epithelial cells In uenza Pathogenesis cont Primary site of infection tracheobronchial tree involving nasopharynx As virus replicates cilia are destroyed Cleaning system in the lungs does not work as well More mucus stays in the airway clogging them causing coughing Destruction of cilia contribute to secondary bacterial pneumonia infections sinusitis otitis U 0 per respiralow respiratory b 3 Alveolar V sacs Nasal cavny Bronchlole Tongue Nasopharynx Eplglol us Gas exchange region Tracheo bronchial tree Tracheal Lung Terminal t 39 if bronchrole Figure 123b Immunity Infected individuals develop antibodies against the outer proteins of Influenza virus neuraminidase N and hemagglutinin H Antibodies against H neutralize the virus Antibodies against N do not but they do reduce the release of virus from infected cells If a person has been infected in the past couple of years by a closely related strain of influenza H subtype Their antibodies may intercept and neutralize the virus protecting the lungs Cytokine Storm Also referred to as systemic inflammatory response syndrome SIRS SIRS may explain the devastating nature of the 1918 strain of influenza The immune system overreacts toward the pathogen Cytokines signal macrophages to travel to the site of infection causing damage to the body and organ failure H5N1 Avian Influenza virus also causes SIRS and is at least 50 lethal in humans 39 H5N1 nfluenzavirus Macrophage becomes a d v quot A Virus replication 5 I and release Viral peptide immunoreceptor T cell Activated macrophage Activated TcelITrs a r39 3 pncontrolled exuberant 2 Immune response Chmoattractants COL 2 Prom ammatory cytokines COL5 ml 9 CJ 0 J g t e Proinflammatory Chemoanractants Cytokines IL6y lbs Proinllamma ory cytokines 0 t 9 to X N V Acute respiratory distress syndrome Necrosis Tissue destruction Influx of leukocytes Dilatation of blood vessels Lungs Viral Replication Properties of the Influenza A Particle Immediately after isolation particles are filamentous Fig 126a After several passages in cell cultures the particles become spherical Fig 126a Figure 126ba Figure 12 Influenza A Virus Particle Enveloped Lipid biiayer 1 Contains hemagglutinin H antigen glycoprotein spikes on the surface and neuraminidase N antigen spikes NA neuraminidase HA hemagglutinin M2 ion channel M1 matrix protein 2 M2 ion channel protein PBLPBZPA Npinucieocapsid transcriptase complex Figure 1260 the influenza A virus structure s D H and N spikes 3 8 segments of ssRNA of negative polarity Virus Nomenclature Influenza type Species isolated form unless human Place of Isolation Strain designation Year isolation HN subtypes Example AHong Kong168H3N2


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