New User Special Price Expires in

Let's log you in.

Sign in with Facebook


Don't have a StudySoup account? Create one here!


Create a StudySoup account

Be part of our community, it's free to join!

Sign up with Facebook


Create your account
By creating an account you agree to StudySoup's terms and conditions and privacy policy

Already have a StudySoup account? Login here

Organic Chemistry Laboratory Biological Emphasis

by: Stephania Powlowski

Organic Chemistry Laboratory Biological Emphasis CHEM 3152

Marketplace > University of Oklahoma > Chemistry and Biochemistry > CHEM 3152 > Organic Chemistry Laboratory Biological Emphasis
Stephania Powlowski
GPA 3.53


Almost Ready


These notes were just uploaded, and will be ready to view shortly.

Purchase these notes here, or revisit this page.

Either way, we'll remind you when they're ready :)

Preview These Notes for FREE

Get a free preview of these Notes, just enter your email below.

Unlock Preview
Unlock Preview

Preview these materials now for free

Why put in your email? Get access to more of this material and other relevant free materials for your school

View Preview

About this Document

Class Notes
25 ?




Popular in Course

Popular in Chemistry and Biochemistry

This 9 page Class Notes was uploaded by Stephania Powlowski on Monday October 26, 2015. The Class Notes belongs to CHEM 3152 at University of Oklahoma taught by Staff in Fall. Since its upload, it has received 35 views. For similar materials see /class/229278/chem-3152-university-of-oklahoma in Chemistry and Biochemistry at University of Oklahoma.


Reviews for Organic Chemistry Laboratory Biological Emphasis


Report this Material


What is Karma?


Karma is the currency of StudySoup.

You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!

Date Created: 10/26/15
General Lab Technigues MP given as a range 122125 Mixtures always have different MP than the pure compound Dry solvents 9 removal of trace amounts of water Drying agent 9 Inorganic salt which takes up water to become hydrated 1 Solubility A compound s ability to dissolve in a specific solvent Units MASS CPDVOL SOLVENT gm L 2 Filtration Technique used to remove solid impurities from an organic solution or to isolate an organic solid 3 Reflux Continuously heating a solvent at it s bp without any loss of the solvent reflux ring is the ring of condensation 4 Recrystallization Used to purify chemicals dissolves crude product in minimal solvent 5 Extraction A separation process consisting of the separation of a substance from a matrix compounds transferred from one phase to another a SOLIDLIQUID i Isolate compounds based on solubilitypolarity ii Liquid extracts certain compounds from solids eg tea coffee b LIQUIDLIQUID i Based on solubility in various immiscible solvents 6 Distillation Technique that separates mixtures based on differences in volatility of components in a boiling liquid mixture 7 Fermentation EtOH or alcoholic fermentation refers to a biological process in which sugars in this case sucrose are converted into cellular energy EtOH and C02 are deemed metabolic waste from this process a SUCROSE INVERTASE 9 GLUCOSE ZYMASE 9 EtOH 8 Azeotrope A mixture of 2 liquids in a way that it s components cannot be altered simply by distillation 9 RECOVERY Mass pureMass crude x 100 10 EXTRACTON Mass pureMass original nutmeg x100 Chromatography Separating chemical mixtures based on a compound s affinity for the stationary polar solid or mobile fluid eluent aka solvent phase Affinities are based on polarity absorptivity solubility size charge 11 Polar Stationary Phase lmmobile immiscible porous polar solid 12 Nonpolar Mobile Phase Fluid moving analytes through the stationary phase nonpolar or weakly polar quoteluentquot quotReverse Phase Chromotographyquot would be a nonpolar stationary phase and a polar mobile phase 13 Thin Layer Chromatography TLC Stationary phase adhered to a plate 14 Column Chromatography Stationary phase packed into a tube Lab 1 Extract on of Nutmeg Nutmeg Spice Mynsuee Tree munu uldry mas Trlmyrlslln Objective recwslalllzallun quotTechniques Extracllun sulldsllquld quotREACIIDN Nutmeg 7 Trimyri trirester triglyceride ryellow gray solid rsoluble in organic nonspolan solvents and insoluble in H20 and pen a l 1 p15 Assume 1 me eua anon umeg mum men yml 3 30m r mmynsm A u eaynellmeu you Wax 39mg ozone pmu lu mm is y i2 win eul 3 l g mnl m eck Shaw lrcm39uyquot Culnunie me uumner nfmults a mmynmn lime moilam in gel rull yams 39mg E Reccv rv 55mg r 4mm m x g x 1 i lDDDnlg 73 leg l in Labd me 3c1ml and lemma yvmuns oi mynsm Ll rm and Jail E39ul rVV D 3 2 pts aneuy eeplem lb elseiee Mdlchlammthnne war 4an as me sulmi acmllkelmwnslln lss g mvgmar rmsauiee ii We Naiensuewuular min max newlysllgnllyoolennmyusun w uursuluulllw HE ul Hkg M an unannmnrlale lvzlll fer The Fermentat on and D 7L u 1 fructose z Zyrhase 2C6H1206 gt 4CZH60 4cm Forms EtOH 1h ah aoueous ehvrro EtOH I nment aho HZO are mfsdb ewhfch rheahs they rhrx 1h 3 orooortrohs Purrtyseoaratezhouros a corhorrseo orz11ouros rbpd139fferentdvanthepur oouhos rh39qm39d vapor ohases havesarhe corhoosm39oh ratro sarhe mo ar ratro We have X W xx H ar oetter 39 39 quot 39 11 1t xv 139s better than xxvv thrs rheahs rt39s a hegatwe azeouooe xw strck together haoorer together than 1h oure forms 2 TVPES 0F AZEDTROPES 1Posmveoo1owerthah the pure cpd EX EtOH H20 9 956 30174496 H20 2 Nega vebp hrgher thah the pure Coos ravorao1e 1 1 pr may can jmu nm purify the emannuoater mummy hysxmple msnumm Erhahm 3511 s 2 3 11 and 39 3r yuu riaHy nee w can a h Wrerumhng Jam and an 2 ulma a Na n u haue m u swam 16 We 1am h snuauuh au 3 Hemrvarwg 33H the zzemroae 2 1 pt mm 1 me pulpns otmappmg alunnnum ml around the gasp mutmon In am amuna to heat raster m M 19mm 1o nsaae a1 a ummer me 37 3 p5 Gwm me equakons far the cmweman orsmnse 1o Edam 11 H o 1 Cold H3011 7 lCuHrOa v4 32H He co Calculate the onuuues nfednnnl 110 719 g39mL pmduned from 17 s gnnls of snubs Assume mpl e comem and Shaw cumaxms m ECEWE full cred u7na1JHA1 Has 13 1 mm 3h u 729g Lab 3 Partition Coefficient The pamlmn uemment is the ram a a phases ExHiqmdshqmd extracuun Keg A organic mol g m A aqueous ml V W w quotunitless rm lgt m quihbrium unslanl sMuiecuies can g0 back and mm between phases Ex 1 ingA m 10ml ulCHZClZ maxI Extracted w m 10ml a Hzo H20 9 u cancemrauuns are Equai Keq 1 ll Keg lt 1 9 mare gradual m ORGANIC If Keg gt 1 9mme graduatinA UEOUS Keq agmomL 4 g 291OmL r m1nml NM Keq4 Aurg mass mg SlamngmassMurgtMaq Alaqu mass aqu Maq start Murg 4 Murg ZsMurg a 16 g Murg w iprmunalmhdeprulunalian 1quot Amd Ease Extractmnquot 0 because yuu uselhemlu Em mmze quot5 I OH v D 24ml mini we mam 3 pkaro whanui enemas n 11 p15 Use a uhwwmg whm mgems m mm m gm hqmdrllqmd exmman aw chm Am each 91 quot91mm 1 mxemcnwu oilwo mm mm Eat b m1 more um mine mm a m M muluyl answu39 p2 my me HChxu NnOHMI CHICKI salvuuruguu ma o camowh and J 9 X Ti 2 WWmmmmmmmmmmm WWW M a w mm WWW pm mmmmmm h D77 xHUmLhexane on mu 7 XJMUmL H20 550 V x m 423 5 1 77x 3 n phane wk nz hum mutant xammg The cundnmn uf bang mssaualen mm mns ma hem radwatmn chemmax reaclmn arerecmcaw mscharge Lab4zf 39 I 39 ovaes 39 9 additionalfxn39l groups 9 Alizarin 0 0 000 OH 0 0 OH Conjugation Poar Yelow Red Separating Dye Mixtures via chromatography separates chemical mixtures analytes based on affinib for stationary or mobile phase Stationary Phase POLAR SOLID uses silica SOZ or alumina ALZOS Mobile Phase NONPOLARWEALY POLAR LIQUID either DCM or acetone 9 quoteluentquot 2 types of chromatography 1 Column Stationary Phase packed in a tube a Separation via graviw b Purification technique can isolate pure cpds c Polar stationary phase polar cpds will stick to it or quotpartition more onto itquot d Nonpolar mobile phase will elude nonpolar compounds first 2 TLC Stationary Phase adhered to a plate plastic metal glass a Separation via capillary action b Stationary phase porous c Eluent travels upthe plate via IMF d Analytical quick method to ID cpds e Polar stick together better nonpolars at the top Retardation Factor RF UNIT Distance traveled by anal e Distance traveled by solvent unique constant for each analyte only true as long as SPMP are the same ie don39t change is used to confirm the identity of a cpd A39E 3 RF A 23 066 cm RFB13033 cm 2m Thus RF lt 1 nonpolar compounds have bigger RF values IfTLC spots are difficult to see use UV lamps conjugation in the 9 1 2 p5 Calculate Imam far cmmpmm s A and B d SlBHCE Irave ed aname 3 d stance Irave ed alven 9 Mm R Az28cm Mam H 3 cm RFB94 m amcm W250 H m mu elm mm ahamam qu Honda 3 msmubk ziplmnm for yam We CJA Separauun wa grauw DEM 5 an wrgarw mommy War and Wm e ude nrgamc munrpman p Anthraumnune y nunweam paw compared In ahzann 1 WM be E UEed nm mu 3 Tu gEV Nd ur av packets a r zddmg sand v a umvna Lab 5 Spectroscopy IR amp MS 3types 1 Infared Spectroscopy IR 2 Mass Spectrometry MS 3 Nuclear Magnetic Resonance Spectroscopy NMR Spectroscopy Study ofthe interactions between electromagnetic radiation EMR and matter 0 Intensity change tells us the molecules are affected by radiation 0 Functional groups always behave the same we can see a trend 3 Presence offunctional groups Uses hv lightradiation to excite a molecule then it goes back to normal Does not tell the relationship to the overall structure Mass Spec Quantification of interactions between EMA and matter Sample hv gt ionsfragments gt analyzer The sample is not recoverable ons are sorted by size 1 mz masscharge ratio 2AssumeZalways 1 mz mw ofthe I 39or the39 quot 39 I 1 Molecular ion m a Loss of a single electron b Peak furthest to the right heaviest to the right lightest to the left 2 Base Peak Tallest peak a Most stable ion 3 Fragmentation a Random but some patterns b Wants to be stable no cations or radicals c MW fragment MW compound MW ion 6 Spectra 4 Halogens a Can differentiate between using mass spec b Naturalabundance c Isotopes H NMR I Allows us to look at the number of protons in the molecule quot Tells us the numbers of protons on each carbon 5 Types of protons a Chemical environment b How neighboring atoms affect protons 4 Relationship of CH groups to one another CHEMICAL SHIFTS Frequency at which the proton shows up at in radiowaves ppm partsmil in a chemical environment Upfield shielded 9 approaches zero Downfield deshielded 6 approaches infinity aky groups at CHx are 0 2 ppm 0 ppm ignore it it s TMS furthest downfield will be your aldehydes and carboxylic acids PEAK INTEGRATION the number next to the peak The area ofthe curve Tes us the number of protons on that carbon Will be given Equivalent protons come out of symmetry eg ethers PEAK SPLITI39ING Show up at multiple peaks Number of neighboring protons CHx connectivity Rue n1 where nnumber of neighboring protons 3 peaks would be 3 n1 so n2 neighboring protons


Buy Material

Are you sure you want to buy this material for

25 Karma

Buy Material

BOOM! Enjoy Your Free Notes!

We've added these Notes to your profile, click here to view them now.


You're already Subscribed!

Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'

Why people love StudySoup

Jim McGreen Ohio University

"Knowing I can count on the Elite Notetaker in my class allows me to focus on what the professor is saying instead of just scribbling notes the whole time and falling behind."

Kyle Maynard Purdue

"When you're taking detailed notes and trying to help everyone else out in the class, it really helps you learn and understand the I made $280 on my first study guide!"

Bentley McCaw University of Florida

"I was shooting for a perfect 4.0 GPA this semester. Having StudySoup as a study aid was critical to helping me achieve my goal...and I nailed it!"


"Their 'Elite Notetakers' are making over $1,200/month in sales by creating high quality content that helps their classmates in a time of need."

Become an Elite Notetaker and start selling your notes online!

Refund Policy


All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email


StudySoup has more than 1 million course-specific study resources to help students study smarter. If you’re having trouble finding what you’re looking for, our customer support team can help you find what you need! Feel free to contact them here:

Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to

Satisfaction Guarantee: If you’re not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.

Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.