New User Special Price Expires in

Let's log you in.

Sign in with Facebook


Don't have a StudySoup account? Create one here!


Create a StudySoup account

Be part of our community, it's free to join!

Sign up with Facebook


Create your account
By creating an account you agree to StudySoup's terms and conditions and privacy policy

Already have a StudySoup account? Login here

BIOL 2170 Chapter Twelve Notes

by: chelsea1023

BIOL 2170 Chapter Twelve Notes BIOL 2170

Marketplace > University of Toledo > Biology > BIOL 2170 > BIOL 2170 Chapter Twelve Notes
GPA 3.6
Fundamentals of Life Science: Biomolecules, cells, and Inheritence
Dr. Deborah Chadee

Almost Ready


These notes were just uploaded, and will be ready to view shortly.

Purchase these notes here, or revisit this page.

Either way, we'll remind you when they're ready :)

Preview These Notes for FREE

Get a free preview of these Notes, just enter your email below.

Unlock Preview
Unlock Preview

Preview these materials now for free

Why put in your email? Get access to more of this material and other relevant free materials for your school

View Preview

About this Document

Summarized lecture notes for chapter twelve
Fundamentals of Life Science: Biomolecules, cells, and Inheritence
Dr. Deborah Chadee
Class Notes
25 ?




Popular in Fundamentals of Life Science: Biomolecules, cells, and Inheritence

Popular in Biology

This 3 page Class Notes was uploaded by chelsea1023 on Tuesday October 27, 2015. The Class Notes belongs to BIOL 2170 at University of Toledo taught by Dr. Deborah Chadee in Summer 2015. Since its upload, it has received 17 views. For similar materials see Fundamentals of Life Science: Biomolecules, cells, and Inheritence in Biology at University of Toledo.


Reviews for BIOL 2170 Chapter Twelve Notes


Report this Material


What is Karma?


Karma is the currency of StudySoup.

You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!

Date Created: 10/27/15
Chapter Twelve DNA Replication and Manipulation DNA Replication 0 Two strands of DNA separate and become parent strands 0 Parent strands serve as templates for creation of new daughter strands o Bases are paired AT and GC 0 Can be conservative or semiconservative In replication DNA is synthesized in the 5 to 3 end Can be continuous or discontinuous replication Proofreading is done after synthesis to ensure that the DNA was translated correctly If not it is changed Replication requires 0 Parent strand 0 DNA polymerase o Topoisomerase o Helicase 0 Nucleotide bases Replication of circular DNA starts at origin and moves around the chromosome in both directions Telomerase lengthens DNA that was previously shortened in DNA replication DNA manipulation 0 PCR polymerase chain reactions O Amplifies a sequence of DNA Heated to separate DNA into two strands denaturation Cooled to allow primers to anneal to their complimentary sequence annealing DNA polymerase creates two new DNA strands in the 5 to 3 direction extension Requires a template DNA DNA polymerase the four nucleotide bases ACTG and two primers Taq polymerase used since it can survive and function at high temperatures Gel Electrophoresis O 0 Samples are loaded into wells in the gel DNA moves toward positive side smaller fragments move farther than larger ones Restriction Digests Enzymes O Cleave DNA Southern Blots 0 Restriction enzyme added Gel electrophoresis Denature DNA and blotted onto filter paper Filter paper is removed after blotting Paper is put in bag containing solution with single stranded probe and probe sticks to complimentary fragments Filter paper is exposed to xray film and the films over each band are darkened Sanger Sequencing 0 Label nucleotides with fluorescent dyes 0 Try to find sequence of daughter strand to determine sequence of parent strand Recombinant DNA similar to crossing over DNA is cleaved and removed so new DNA can be added in its place


Buy Material

Are you sure you want to buy this material for

25 Karma

Buy Material

BOOM! Enjoy Your Free Notes!

We've added these Notes to your profile, click here to view them now.


You're already Subscribed!

Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'

Why people love StudySoup

Bentley McCaw University of Florida

"I was shooting for a perfect 4.0 GPA this semester. Having StudySoup as a study aid was critical to helping me achieve my goal...and I nailed it!"

Kyle Maynard Purdue

"When you're taking detailed notes and trying to help everyone else out in the class, it really helps you learn and understand the I made $280 on my first study guide!"

Jim McGreen Ohio University

"Knowing I can count on the Elite Notetaker in my class allows me to focus on what the professor is saying instead of just scribbling notes the whole time and falling behind."

Parker Thompson 500 Startups

"It's a great way for students to improve their educational experience and it seemed like a product that everybody wants, so all the people participating are winning."

Become an Elite Notetaker and start selling your notes online!

Refund Policy


All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email


StudySoup has more than 1 million course-specific study resources to help students study smarter. If you’re having trouble finding what you’re looking for, our customer support team can help you find what you need! Feel free to contact them here:

Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to

Satisfaction Guarantee: If you’re not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.

Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.