Introductionto Biochemistry CHEM 501
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This 7 page Class Notes was uploaded by Mrs. Francis Runolfsson on Tuesday October 27, 2015. The Class Notes belongs to CHEM 501 at University of Wisconsin - Milwaukee taught by Staff in Fall. Since its upload, it has received 10 views. For similar materials see /class/230296/chem-501-university-of-wisconsin-milwaukee in Chemistry at University of Wisconsin - Milwaukee.
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Date Created: 10/27/15
a E UNIVERSITYMWISCONSIN MILWAUKEE Dep of Chemistry amp Biochemistry Albert Lehninger Prof Ihdig 191 71 986 I Chemistry 501 Handout 19 Oxidative Phosphorylation and Photophosphorylation Chapter 19 Treatment with digilonin Lehninger Principles of Biochemistry by Nelson and Cox 5 Edition WH Freeman and Company Biochemical anatomy of a mitochondrion Outer membrane Freely permeable to I small molecules and ions ATP synthase F0F1 Inner membrane lmpermeable to most small molecules and ions including H on ains Respiratory electron carriers Complexes l IV ADPATP translocase ATP synthase F0F1 Contains Pyruvate dehydrogenase com plex Citric acid cycle enzymes Fatty acid oxidation I zymes Amino acid oxidation enzymes Porin channels DNA ribosomes Many other enzymes ATP ADP Pi Mg Ca K Many soluble metabolic intermediates Separation of functional complexes of the respiratory chain Treatment with digitonin membrane fragments Outer membrane 39 fragments discarded Treatment with digitonin Separation of functional complexes ofthe respiratory chain fragments Outer membrane fragm dlscarded ATP 1 synthase Solubilization with detergent f quotowed by ionexchange chromatography IV ATP synthasiI 5quotquot 7 1 L m HrW r n r A A A A 39 Q Cyt Cytc 02 ATP ADP Pi Reactions catalyzed by isalated actions in vitro Enzyme complexprotein Mass k Da Number of subunits Prosthetic groups I NADH dehydrogenase 850 43 14 FMN FeS ll Succinate dehydrogenase 140 4 FAD FeS Ill Ubiquinonecytochrome c oxidoreductase 250 1 1 Hemes FeS Cytochrome cf 13 1 Heme IV Cytochrome oxidase 160 13 3 4 Hemes Cu A CuB Numbers of subunits in the bacterial equivalents in parentheses fCytochrome c is not part of an enzyme complex it moves between Complexes Ill and IV as a freely soluble protein Solubilization with detergent followed by ionIexchange chromatography 111 III IV ATP synthase IKE 39 fIquot Reactions catalyzed by isolated Pi fractions in vitro H3 0 CH30 CH 2 CH C CH21 0 H Ubiquinone Q CH 30 CH 3 fully oxidized 0 1 Hquot e39 O CH30 R Semiquinone radical OH CH 30 CH 3 NADH dehydrogenase complex I NADH ubiquinone oxidoreductase Intermembrane 4H space P side Complex OH P m 939 Matrix N side OH CH30 R Ubiquinol OH 2 fully reduced CH30 CH 3 0H 7 NADH H Series mm of FeS centers 8 b Cys Cys Cys 3 w i cys cys Cys 1 Protein
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