Adv Clinical Immunohemotology
Adv Clinical Immunohemotology CLS 3311
Weber State University
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This 3 page Class Notes was uploaded by Mr. Carter Bogisich on Wednesday October 28, 2015. The Class Notes belongs to CLS 3311 at Weber State University taught by Staff in Fall. Since its upload, it has received 13 views. For similar materials see /class/230773/cls-3311-weber-state-university in Clinical Laboratory Sciences at Weber State University.
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Date Created: 10/28/15
CLS 3311 Advanced Immunohematology Objectives for Rh blood group system lecture The student will have the opportunity to 1 Compare the theories of inheritance of the Rh system in respect to the investigators terminology s genetic control and conformation to current theories Describe the current proposed genetic theory of the Rh system Describe the characteristics of the rst ve Rh system antigens and their antibodies with regard to enzyme treatment dosage complement binding reaction temperature pH enhancement media immunoglobulin class placental passage clinical signi cance and numberlocation of antigen sites on cell Convert from the FisherRace to the Weiner nomenclature and vice versa Compare and contrast the three mechanisms explaining the Weak D antigen Explain why knowing the D status is required in donor testing and not required in recipient testing Interpret Rh antisera typing results D C c E e in both Wiener and FisherRace nomenclature and using the phenotype frequencies indicate the most probable genotype include any additional possible genotypes when requested Describe the reagents used for detection of the D antigen including monoclonal blend monoclonalpolyclonal blend high protein and chemically modi ed indicating the purpose and contents of the control for each reagent Identify the frequency of the ve major Rh antigens List the frequencies of the ve most common Rh haplotypes for White Black and Asian populations Compare and contrast the following Rh antigens according to de nition frequency antigenic reactivity etc G DC f ce D Ce C V and VS Analyze the possible causes of a positive Rh control and why this invalidates the Weak D test Examine the Rhnull phenotype identifying the several genetic explanations antigen expression peripheral blood ndings etc Explain Rh Immune Globulin according to its content function and use List the rst ve most common Rh haplotypes in order of frequency using the FisherRace Weiner and Rosenfeld nomenclatures CLS DE PT SAHSUTMB IMMUNOHEMATO LOGY c ST JOHN CLS 3311 Advanced Immunohematology ABO Blood Group lecture objectives The student will be given the opportunity to 1 2 Diagram the steps in the production of the H A amp B antigens beginning with the precursor substrate and including the action of the H A B amp 0 genes Differentiate the oligosaccarhide precursor chains found in the secretions versus those found on the RBC membrane Illustrate the effect the Secretor genes have in the production of H A B amp Lewis Ag s in the secretions Compare and contrast the result of inheriting an quotHquot gene versus a homozygous quothquot gene Given the ABO H amp Lewis Ag39s present on the RBC s and in the secretions select the most probable genotype of the individual Given the A B O H Le and Secretor genes inherited determine the antigens present in the secretions and on the RBC membrane Given the ABO phenotype select the most probable genotype considering the gene frequencies within the general population Compare amp contrast the Subgroups of A and B A1 A2 A3 Ax B B3 and Bx in terms of efficiency of antigen production tendency to produce antiA1 or AntiB and strength of reactions with antiA antiA1 antiAB and A1 A2 and B cells Given the results of ABO cell amp serum groupings interpret the ABO group of the specimen Compare and contrast the forward amp reverse ABO grouping in terms of what is being tested in the serum amp on the RBC s Explain why there is a reverse grouping for the ABO system as compared to other blood group systems Interpret appropriately the results of the forward amp reverse ABO grouping Given the results of forward amp reverse groupings correctly differentiate those which have normal results from those that have abnormal reactions Given the results of forward amp reverse groupings identify the most probably abnormal reactions amp indicate the possible causes Given the results of an abnormal ABO grouping as well as the possible causes determine what followup testing is most appropriate to resolve the problem