Intro CLS 1113
Weber State University
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This 2 page Class Notes was uploaded by Mr. Carter Bogisich on Wednesday October 28, 2015. The Class Notes belongs to CLS 1113 at Weber State University taught by Staff in Fall. Since its upload, it has received 15 views. For similar materials see /class/230774/cls-1113-weber-state-university in Clinical Laboratory Sciences at Weber State University.
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Date Created: 10/28/15
Clinical Laboratory Sciences 1113 Introduction to Clinical Laboratory Practices UNIT 5 IMMUNOLOGIC PROCEDURES LEARNING OBJECTIVES At the completion of this Module the student will be able to 1 2 V39 0 De ne key terms for list below Illustrate how the Law of Mass Action requires exteran factors to visualize AgAb reactions in vitro Discuss affinity and avidity and relate the terms to their in uence on AgAb reactions De ne and describe the principles of turbidimetry and nephelometry Explain the difference between double and single diffusion testing methods Describe the test principle reagents time requirements and limitations of Radioimmunodiffusion Differentiate between agglutination and precipitation Compare the two stages of agglutination and the factors affecting each stage Describe the test principle reagents time requirements and limitations of Immunoelectrophoresis Define and describe the principle of the following competitive and noncompetitive labeled antibody methods Describe how each are used to identify either an antigen or antibody EnzymeImmunoassay EIA Radioimmunoassay RIA Micropartical Enzyme Immunoassay MEIA Direct and IndirectIimmuno ourescent Assasy IFA Chemiluminesent Immunoassay rap96x List common problems encountered with each above procedure and describe techniques that are utilized to help resolve these problems Given hypothetical situations in which you must analyze for an antigen andor antibody design a procedure utilizing any principle presented in this unit LEARNING ACTIVITIES 1 Read Stevens Chapters 810 as guided by course objectives Chapter review highlights 2 Attend lecture and discussions of objectives 3 Compile reading and lecture notes to answer each objective KEY TERMS Chapters 8 10 Af nity Agglutination Agglutination Inhibition Avidity Chemiluminescence Complement xation Direct Agglutination Test DAT Electrophoresis Enzyme Linked Immunosorbent assay ELISA Hemagglutination Immunoelectrophoresis Indirect Antiglobulin test Lattice Formation agglutination Law of Mass Action Ligand Nephelometry Ouchterlony Passive Agglutination Postzone Prozone Precipitation Radioimmunoassay RIA Radial Immunodiffusion Sensitization as it relates to agglutination Zone of equivalence
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