Instrumental Analysis and Bioanalytical Chemistry
Instrumental Analysis and Bioanalytical Chemistry CHEM 115
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This 30 page Class Notes was uploaded by Mason Towne on Thursday October 29, 2015. The Class Notes belongs to CHEM 115 at University of California - Merced taught by Erik Menke in Fall. Since its upload, it has received 16 views. For similar materials see /class/231727/chem-115-university-of-california-merced in Chemistry at University of California - Merced.
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Date Created: 10/29/15
Chem 115 Instrumental Analysis and Bioanalytical Chemistry httpfacultyucmercededuemenkeChem115f09indexhtm Chem 115 Instrumental Analysis and Bioanalytical Chemistry This is the course syllabus for Chemistry 115 the instrumental analysis and bioanalytical chemistry class at UC Merced A note about printing this page Please don t This page will be at this URL long enough to share with your children and grandchildren You don t need a paper copy of it Instructor Professor Erik Menke Class Room and Hours COB 209 MWF 9 950 am Of ce Science and Engineering Room 358 Of ce Hours Drop by my office any time if you have questions or concerns If I don t have time to talk we can schedule a time In addition I will be available for the halfhour immediately following class at the Lantern Caf in the library Contacting me In addition to Visiting my of ce you can reach me by e mail or leaving a message on the UCMCrops website for the class Course description and objectives Analytical chemistry is one of the broadest disciplines of chemistry with the primary goal of developing the tools and experiments to either qualitatively or quantitatively measure chemical composition Historically analytical chemistry was primarily performed by wet methods most notably titrations but over the last decade instrumental tools and techniques have come to dominate the field This course focuses on how these instruments work and what samples they are best suited for The instruments and experiments we will look at are FTIR FT NMR UV Vis GC HPLC AAS and AES and electrophoresis Expected Student Learning Outcomes By the end of the semester you should be able to Identify the most useful technique and instrument for a given analytical problem Identify the different parts of analytical instruments and explain how they work Calculate relative and absolute amounts of an unknown substance from instrumental data as well as the error in the measurements Required Text Undergraduate Instrumental Analysis by James W Robinson Eileen M Skelly Frame and George M Frame II Sixth Edition Prerequisites Chem 112 which may be taken concurrently Class Policies The number one rule is to respect the time of everybody in the class including the instructor Ultimately I believe that this is the only rule we should need but it is rather vague To help overcome the vagueness of said rule here are some examples of what this covers Refrain from using cell phones in class this includes texting or having the ring volume above vibrate Refrain from talking out of turn Do not tease taunt or belittle others Anything that someone else reads e mails homework message posts should be legible with mostly proper grammatical structure and spelling 1 392 http facuty ucmerced eduemenkeChem115 f09index html Academic Integrity This is a topic that I take very seriously Mlle I understand that shortens m attrsotrye 39 39 39 39 39 l x L a n J n v be founcl or polley will be dealt with harshly r rr r r 1 11139 Tenetng help a information on then webslte ermalling them at dls 39 39 d or erlllhg them at quot0039 R uo I MM effect or your ability to understh the material Bums or r r r r L v n will be 39 doctor s note r 1 L L will be worth 75 points eaeb Emu Schedule Exam 2 Friday Oetober 2 Exam 2 Fn39day November 6 Exam 3 Wednesday December 9 Preeematlon Friday December 1 from Ben am Homework There wxll be two kinds of homework problems erdw y rr y r or class but they will not be graded Exam problems are typica y of this dif culty u s gt A n l A A There will be 39 earbwelr d39 quot391 I H 1 t Meal 39 39 the rm V r h n inquot rquot llnirirl Grading Overall there will be a total of 300 points so palms for eaeb exam 50 pains for the 7 h r A so 7 Bo B 79 7 7o cm with any ro be le fordle inmucmrs daseretror http faculm ucmerced eduemenkeChem115 f09index html Tlus is a tellta ve schedule for the class Hou even as this class has never been taught before deviations from this schedule are likely Week Lecture at Week of Topics Chapterls Lecture t t Lemme 2 August 26m Concepts data enatysrs I Lecture 3 2 Lecture 4 August 31st Sample prep sotutron chermstry 1 Lecture 5 Lecture 5 3 Ledure 7 September 9m LIghLmatter mteractron optrcs 2 Lecture 5 4 Lecture 9 September Mm NMR 3 Lecture to 5 Lemme M September 2151 243 NMR solrd state NMR 3 Lecture t2 Lecture 13 6 Lecture 14 September 25m tR spectroscopy 4 Exam 1 Lecture t5 7 Lecture t6 October an R spectroscopy 4 Lecture 17 Lecture quot8 5 Lecture 19 October 12m UVths Fluorescence 5 Lecture 20 Lecture 21 9 Lecture 22 October 19m Atomrc absorptron 6 Lecture 23 Lecmre October 26m Alomlc emrsslbn Lecmre Lecmre Exam 2 November 2nd Mass Spectrometry Lecmre Lecmre November 901 Gas Chromamgraphy Lecmre Lecture Lecmre November LBth quurd Chromatography Lecmre Lecmre November 23rd Eleurophoresrs Lecmre November 30m eray techniques Lecmre Exam 3 December 7th eray techniques 0 I will post these lectures as a pdf on the website after each Class 0 So when taking notes make sure to write down what I m saying that DOESN T appear on the slides 0 Each slide has a unique serial number so if you have a question about a slide refer to that number 0 For example this slide is number 17 Lecture number 0 For example this slide is number 18 Slide number Chem 115 Instrumental Analysis and Bioanalytical Chemistry Part 1 Concepts and analysis What s in this lecture 0 What is analytical chemistry 0 How do analytical chemists approach problems What is analytical chemistry The qualitative and quantitative characterization of matter 0 Qualitative What is this 0 Quantitative How much is there Analytical chemistry is broad Pharmaceuticals Manufacturing Law enforcement Museums Why instrumental analysis Faster than wet methods More sensitive Less operator error Typically nondestructive What goes into instrumental analysis Chemistry Physics Electronics Biology Materials science Food science It is the analytical Chemist s job to communicate with other scientists 1 2 3 4 5 6 7 8 The analytical approach Define the problem Design the method Sampling and sample storage Sample preparation Perform the measurement Assess the data Validate the method Document Define the problem How precise and accurate Organic inorganic or both Possible interferences Qualitative or quantitative Qualitative analysis 0 What is this 0 Distinguish between molecular analysis and elemental analysis 0 Molecular Sucrose vs Fructose 0 Elemental Carbon vs Oxygen Quantitative analysis 0 How much is there 0 Generally expressed as a concentration 0 Sensitivity is important Concentration 0 Molarity moles per liter 0 Percent 0 Atomic percent 0 Weight percent 0 Volume percent 0 Parts per 0 Hundred same as 0 Thousand 0 Million 0 Billion 0 Trillion Concentration cont 0 Molality moles per gram 0 Mole fraction 0 Normality Molarity vs molality 0 Molarity 0 Molality 0 Depends on mass 0 Depends on mass and volume only 0 Temperature 0 Temperature dependent independent Common instrumental methods Method Qualitative Quantitative Elemental Molecular Elemental Molecular Atomic absorption No No Yes No Atomic emission Yes No Yes No Capillary electrophoresis Yes Yes Electrochemistry Yes Yes Gas chromatography No No lCPMass spectrometry Yes Yes IR spectroscopy No No lon chromatography Yes Yes Liquid chromatography No No Mass spectrometry Yes Yes Nuclear magnetic resonance No No Raman spectroscopy No No Thermal analysis UVVis spectroscopy UV absorption UV fluorescence X ray absorption Xray diffraction X ray fluorescence Concentration ranges Ultratrace Trace Minor Major lt1 ppm 1ppm 01 01 10 gt10 Xray diffraction No No No Yes Yes N M R No No Yes Yes Yes X ray fluorescence No No Yes Yes Yes Method Destructive IR spectroscopy No No Yes Yes Yes Raman spectroscopy No No Yes Yes Yes UVVl S No No Yes Yes Yes Colorimetry No Yes Yes No Molecular fluorescence No Yes Yes Yes Atomic absorption Yes Yes No Atomic emission Yes Yes Yes lCPMS Yes Yes No GCMS Yes Yes Yes LCMS Yes Yes Yes Potentiometry Yes Yes Yes Voltammetry Yes Yes Yes H PLC Yes Yes Yes lon chromatography Yes Yes Yes Capillary electrophoresis Yes Yes Yes Thermal analysis No Yes Yes 1 2 3 4 5 6 7 8 The analytical approach Define the problem Design the method Sampling and sample storage Sample preparation Perform the measurement Assess the data Validate the method Document Defining the problem 1 What accuracy is required 2 How much sample is available 3 What is the concentration range 4 What are likely interferences 5 What are the matrix properties 6 How many samples Designing the analytical method lelfijne 0 Sample 0 EHampS 0 Blank 0 Matrix 0 Reagent 0 Standard 0 Literature 0 Journals 0 Books 0 Associations 1 2 3 4 5 6 7 8 The analytical approach Define the problem Design the method Sampling and sample storage Sample preparation Perform the measurement Assess the data Validate the method Document Sampling 0 Need a sample that is representative 0 Worry about contamination 0 Many standard methods The analytical approach lDefine the problem ZDesign the method ZSampling and sample storage 4 Sample preparation 5 Perform the measurement 6 Assess the data 8 Deeument