MOLECULAR BIOLOGY MCDB 3500
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This 1 page Class Notes was uploaded by Mrs. Willis Mante on Thursday October 29, 2015. The Class Notes belongs to MCDB 3500 at University of Colorado at Boulder taught by Staff in Fall. Since its upload, it has received 24 views. For similar materials see /class/231845/mcdb-3500-university-of-colorado-at-boulder in Molecular, Cellular And Developmental Biology at University of Colorado at Boulder.
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Date Created: 10/29/15
MCDB 3500 Lecture 4 12302 Weaver Figures 2028 2029 2030 2017 Common enzymology terms DNA polymeraseisynthesizes DNA DNAdependent 0r RNAdependent RNA polymeraseisynthesizes RNA DNAdependent 0r RNAdependent Primaseisynthesizes RNA primers on DNA DNaseiDegrades DNA RNaseiDegrades RNA ExonucleaseiDegrades polynucleotides from the ends EndonucleaseiBreaks the 3 5 phosphodiester bonds within the polynucleotide chain LigaseiLigates polynucleotides DNA or RNAspeci c Helicaseiseparates strands Top0is0merasesIntr0duces transient DNA breaks Type I makes a single strand cut Type 11 makes a double strand cut Enzymology of DNA Replication in E coli 1 Primase DNA primaseiDNA dep RNA polymerase 2 DNA polymerases DNA polymerase I Single protein 5 7gt 3 DNA polymerase activity 3 gt 5 Exonuclease actiVity 5 7gt 3 Exonuclease actiVity DNA Polymerase III 10 subunits 5 7gt 3 DNA polymerase actiVity 3 gt 5 Exonuclease actiVity In Eukaryotes Table 204 DNA pol OL synthesis of lagging strand primase actiVity DNA pol 6 synthesis of leading strand Highly processive 3 Single Stranded DNA Binding SSB Proteins Binds to ssDNA and keeps it from making ds DNA Stimulates DNA polymerases Essential for DNA replication Not much is known about the eukaryotic SSBs 4 DNA Helicases 4 Found in E coli DnaB ATPases associated with Primase Unwinds DNA double helix during DNA replication strand separation Q H ow does one assay for the DNA helicase activity
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