Cell culture Set 3 of 13
Cell culture Set 3 of 13 A214
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This 2 page Class Notes was uploaded by Tarn Notetaker on Monday November 16, 2015. The Class Notes belongs to A214 at Republic Polytechnic taught by Souvik Chatterji in Fall 2015. Since its upload, it has received 23 views. For similar materials see Cell Culture in Natural Sciences and Mathematics at Republic Polytechnic.
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Date Created: 11/16/15
Subculture cells: Prevents deterioration of cells Prevents spontaneous transformation of primary cells Maintains good cell growth Method Washing, splitting and reseeding Use trypsin or cell scraper Splitting cells using appropriate split ratio Dilution of cells in original flask into a new flask Reseed cells in flask/ seed new flask Passage number Number of times a cell line of subcultured Morphological changes after several passages Cell population doubling time progressively increases until proliferation eventually ceases Spontaneous cellular transformation may occur with extended time in culture Confluency: Measure of cell growth in terms of surface area of the culture dish that is covered by cells 100% confluency means that there is no room on the dish for cells to grow Split ratio: Chosen to obtain confluent cells over an estimated period of culture Example: 1:20 1 part cell suspension + 19 parts media 1:10 1 part cell suspension + 9 parts media 1:5 1 part cell suspension + 4 parts media