Lecture Notes 4-5-16 Jyoti
Lecture Notes 4-5-16 Jyoti BIO 2600
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This 3 page Class Notes was uploaded by Markiesha Notetaker on Tuesday April 5, 2016. The Class Notes belongs to BIO 2600 at Wayne State University taught by Dr. Jyoti Nautiyal in Winter 2016. Since its upload, it has received 554 views. For similar materials see Intr To Cell Biology in Biology at Wayne State University.
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Date Created: 04/05/16
Cell Bio lecture 4-5-16 11, 12, 15 and 16 will be on exam 3. Review slides for exam 2 Chapter 15- intracellular compartments and transport. (Chapter 11) Cell surface modification—ex) blood typing, cell surface markers for cancer cells Chapter 12- pumps and channels Potassium and sodium pump = voltage gated channels Action potentials unidirectional flow is due to inactivation of sodium channels (absolute refractory phase) Neurotransmitter –Ach acetylcholine, GABA.. Inhibitory/excitatory neurotransmitter – o Stimulant increase possibility of action potentials o Inhibitory- decreases possibility of action potential by causing depolarization Clicker question Membrane proteins perform Anchoring Receptors Recognition Enzymes Not Regulation of gene transcription. Clicker question Organelle that modifies and packages newly synthesized proteins – rough endoplasmic reticulum Chapter 15 Notes Membrane enclosed organelles Eukaryotic DNA in nucleus DNA mRNA mRNA modified and sent to cytoplasm mRNA protein synthesis Enzymes need to be sent to specific places. This chapter tells how they are transported and where to. Membrane enclosed organelles History: Ancient prokaryote (just has DNA in an area called nucleoid) Ancient eukaryote- membrane pinched off and made compartment where DNA is (now nucleus) Mitochondria was originally an aerobic prokaryotic cell that was engulfed by a eukaryotic cell Clicker question Order for protein destined for plasma membrane is: ER Golgi Apparatus Plasma Membrane ** Study Slide 12 in this lecture (chapter 15) Mechanisms of transport 1. Transport through nuclear pores a. Import into nucleus examples (histones, transcription factors, DNA polymerase) b. Export out of nucleus examples (regulatory transcription factors) c. Proteins are folded (not linear) during this transport because the nuclear pores are big enough to fit. 2. Membrane transport a. Proteins must be linear (not folded) in order to get through the membrane 3. Vesicles by Endoplasmic reticulum a. These proteins are then transported to the Golgi Apparatus When proteins are synthesized, the default action is to secrete them. The only way to stay in the ER is to have a retention sequence. (See slide with tables of sequences. You do not need to memorize sequences, only specific information listed next) *** Retention (to stay in the ER) sequence in protein must be present at C-terminus (COOH-). The sequence is denoted as KDEL. *** For the protein to be imported into the ER, the protein must have a certain sequence at the N-terminus. Nuclear Pores Transportation (See Slide) 1. Protein needs nuclear localization signal 2. The import receptor binds to the protein 3. The receptor brings it into the nucleus *** Read book on Ran-GTP (skip details, know the basics) Membrane Transport (See slide) 1. SRP recognizes protein by signal sequence on 5’ (N-terminus) end 2. SRP binds to SRP receptor 3. Receptor gives protein to protein translocator 4. The protein translocator pushes the protein into the cell 5. Protein loses signal sequence due to signal peptidase 6. Translocation channel closes 7. If the protein has retention sequence, it stays in the ER. If it does not have the retention sequence, it gets shipped out of the ER.
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