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Get Full Access to Quantitative Chemical Analysis - 8 Edition - Chapter 4 - Problem 4-g
Get Full Access to Quantitative Chemical Analysis - 8 Edition - Chapter 4 - Problem 4-g

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# Calibration curve. (You can do this exercise with your calculator, but it is more easily ISBN: 9781429218153 475

## Solution for problem 4-G Chapter 4

Quantitative Chemical Analysis | 8th Edition

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Problem 4-G

Calibration curve. (You can do this exercise with your calculator, but it is more easily done by the spreadsheet in Figure 4-15.) In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to protein. Absorbance of light is measured. Protein (g): 0.00 9.36 18.72 28.08 37.44 Absorbance at 595 nm: 0.466 0.676 0.883 1.086 1.280 4 (a) Find the equation of the least-squares straight line through these points in the form y [m(sm)]x [b(sb)] with a reasonable number of significant figures. (b) Make a graph showing the experimental data and the calculated straight line. (c) An unknown protein sample gave an absorbance of 0.973. Calculate the number of micrograms of protein in the unknown and estimate its uncertainty.

Step-by-Step Solution:
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2.7 Density  Density is basically the relative heaviness of an object  The formula is D = m/v  A deviation that might have to be used is M = D x V  Density is a physical property of matter.  The density of water @ 4 ° Celsius is 1.00 g/ml  Use density to calculate mass from volume Example 1: Mercury has a density of 13.6 g/ml. What size bottle will contain 13.6 g/ml 13.6 x 1ml = 8.35 ml 13.6 g 3 Example 2: Gold has a density of 19.3 g/ cm . What’s the mass of a gold cube with 1.27 cm sides (a ½ inch cube). ** This problem will use the deviation I listed in the bullets above** cm V = (1.27 ¿¿3 = 2.05 3 cm

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##### ISBN: 9781429218153

The full step-by-step solution to problem: 4-G from chapter: 4 was answered by , our top Chemistry solution expert on 03/16/18, 04:33PM. This textbook survival guide was created for the textbook: Quantitative Chemical Analysis, edition: 8. Since the solution to 4-G from 4 chapter was answered, more than 352 students have viewed the full step-by-step answer. The answer to “Calibration curve. (You can do this exercise with your calculator, but it is more easily done by the spreadsheet in Figure 4-15.) In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to protein. Absorbance of light is measured. Protein (g): 0.00 9.36 18.72 28.08 37.44 Absorbance at 595 nm: 0.466 0.676 0.883 1.086 1.280 4 (a) Find the equation of the least-squares straight line through these points in the form y [m(sm)]x [b(sb)] with a reasonable number of significant figures. (b) Make a graph showing the experimental data and the calculated straight line. (c) An unknown protein sample gave an absorbance of 0.973. Calculate the number of micrograms of protein in the unknown and estimate its uncertainty.” is broken down into a number of easy to follow steps, and 126 words. Quantitative Chemical Analysis was written by and is associated to the ISBN: 9781429218153. This full solution covers the following key subjects: . This expansive textbook survival guide covers 55 chapters, and 1224 solutions.

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