Spectrophotometry with indicators. Acid-base indicators are themselves acids or bases
Chapter 10, Problem 10-K(choose chapter or problem)
Spectrophotometry with indicators. Acid-base indicators are themselves acids or bases. Consider an indicator, HIn, which dissociates according to the equation The molar absorptivity, , is 2 080 M1 cm1 for HIn and 14 200 M1 cm1 for In, at a wavelength of 440 nm. (a) Write an expression for the absorbance of a solution containing HIn at a concentration [HIn] and In at a concentration [In] in a cell of pathlength 1.00 cm. The total absorbance is the sum of absorbances of each component. (b) A solution containing indicator at a formal concentration of 1.84 104 M is adjusted to pH 6.23 and found to exhibit an absorbance of 0.868 at 440 nm. Calculate pKa for this indicator.
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