What are the general steps in developing an isocratic separation for reversed-phase chromatography with two organic solvents?
I. Chapter 11 Continued A. Concentration Units 1. Molarity (M) a. M = moles/liters b. [KCl] means molarity of a KCl solution 2. Molality (m) a. m = moles/kg of solvent b. mass-based and independent of temperature 3. Mass Fraction a. Mass fraction = mass of solute/total mass b. Mass percent = mass fraction * 100% 4. Mass Percent a. Mass percent is parts per hundred i. Ppm = part per million ii. Ppb = parts per billion iii. Ppt = parts per trillion b. 1 ppm of solute in water = 1 mg/1000g of solution