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Solved: (a) Make a graph showing the retention time of each peak in Figure 24-25 in

Quantitative Chemical Analysis | 8th Edition | ISBN: 9781429218153 | Authors: Daniel C. Harris ISBN: 9781429218153 475

Solution for problem 24-34 Chapter 24

Quantitative Chemical Analysis | 8th Edition

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Quantitative Chemical Analysis | 8th Edition | ISBN: 9781429218153 | Authors: Daniel C. Harris

Quantitative Chemical Analysis | 8th Edition

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Problem 24-34

(a) Make a graph showing the retention time of each peak in Figure 24-25 in chromatograms B, F, and C as a function of position along the line BC. Predict the retention times for solvent compositions midway between B and F and midway between F and C. Draw a stick diagram (representing each peak as a vertical line) of each of the two predicted chromatograms. (b) What would be the solvent compositions midway between B and F and midway between F and C?

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C127 Lecture 8 Notes- Bioanalytical Spectroscopy 3-10-16  Learning Goals o Learn principles of UV-Visible absorption spectroscopy o Learn how absorption of light is related to concentration (Beer’s Law) o Use spectroscopy to determine concentration of a chemical (whey protein) in milk without precipitating anything  Add stain/dye to protein to cause color change; whey is colorless by itself o For this dye, has different color in polar surroundings than nonpolar  Safety o Wear gloves  Protein standard has small amount of toxic chemical (sodium azide)  Bradford reagent contains phosphoric acid, which is corrosive, and will stain skin blue

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Chapter 24, Problem 24-34 is Solved
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Textbook: Quantitative Chemical Analysis
Edition: 8
Author: Daniel C. Harris
ISBN: 9781429218153

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Solved: (a) Make a graph showing the retention time of each peak in Figure 24-25 in