A mixture of 14 compounds was subjected to a reversedphase gradient separation going

Chapter 24, Problem 24-38

(choose chapter or problem)

A mixture of 14 compounds was subjected to a reversedphase gradient separation going from 5% to 100% acetonitrile with a gradient time of 60 min. The sample was injected at t dwell time. All peaks were eluted between 22 and 41 min. (a) Is the mixture more suitable for isocratic or gradient elution? (b) If the next run is a gradient, select the starting and ending %acetonitrile and the gradient time.

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