(a) Explain how anions and cations can be separated by hydrophilic interaction
Chapter 25, Problem 25-10(choose chapter or problem)
(a) Explain how anions and cations can be separated by hydrophilic interaction chromatography in Figure 25-5, using the zwitterionic stationary phase in Figure 24-14. Why does the gradient go from high CH3CN to low CH3CN content? (b) Isocratic elution with 20 vol% acetonitrile plus 80 vol% aqueous buffer did not separate Na from Cl . Eluent with 40 vol% acetonitrile plus 60 vol% aqueous buffer gave a baseline separation. Suggest an explanation.
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