Solution Found!
A gel-filtration column has a radius, r, of 0.80 cm and a length, l, of 20.0 cm. (a)
Chapter 25, Problem 25-18(choose chapter or problem)
A gel-filtration column has a radius, r, of 0.80 cm and a length, l, of 20.0 cm. (a) Calculate the volume, Vt, of the column, which is equal to r 2 l. (b) The void volume, Vo, was 18.1 mL, and the total volume of mobile phase was 35.8 mL. Find Kav for a solute eluted at 27.4 mL.
Questions & Answers
QUESTION:
A gel-filtration column has a radius, r, of 0.80 cm and a length, l, of 20.0 cm. (a) Calculate the volume, Vt, of the column, which is equal to r 2 l. (b) The void volume, Vo, was 18.1 mL, and the total volume of mobile phase was 35.8 mL. Find Kav for a solute eluted at 27.4 mL.
ANSWER:Step 1 of 3
(a)
Volume of the column can calculated by the following formula;
Here,