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A gel-filtration column has a radius, r, of 0.80 cm and a length, l, of 20.0 cm. (a)

Chapter 25, Problem 25-18

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QUESTION:

A gel-filtration column has a radius, r, of 0.80 cm and a length, l, of 20.0 cm. (a) Calculate the volume, Vt, of the column, which is equal to r 2 l. (b) The void volume, Vo, was 18.1 mL, and the total volume of mobile phase was 35.8 mL. Find Kav for a solute eluted at 27.4 mL.

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QUESTION:

A gel-filtration column has a radius, r, of 0.80 cm and a length, l, of 20.0 cm. (a) Calculate the volume, Vt, of the column, which is equal to r 2 l. (b) The void volume, Vo, was 18.1 mL, and the total volume of mobile phase was 35.8 mL. Find Kav for a solute eluted at 27.4 mL.

ANSWER:

Step 1 of 3

(a)

Volume of the column can calculated by the following formula;

                                                           
Here,



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