Solved: Citrate synthase, one of the enzymes in the series of enzyme-catalyzed reactions

Chapter 4, Problem 80

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Citrate synthase, one of the enzymes in the series of enzyme-catalyzed reactions known as the citric acid cycle (Section 24.9), catalyzes the synthesis ofcitric acid from oxaloacetic acid and acetyl-CoA. If the synthesis is carried out with acetyl-CoA that contains radioactive carbon (14C) in the indicatedposition(Section 1.1), the isomer shown here is obtained.a. Which stereoisomer of citric acid is synthesized: R or S?b. If the acetyl-CoA used in the synthesis contains 12C instead of 14C, will the product of the reaction be chiral or achiral?

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