Determine the amino acid sequence of a polypeptide from the following data:Complete
Chapter 21, Problem 59(choose chapter or problem)
Determine the amino acid sequence of a polypeptide from the following data:Complete hydrolysis of the peptide yields Arg, 2 Gly, Ile, 3 Leu, 2 Lys, 2 Met, 2 Phe, Pro, Ser, 2 Tyr, and Val.Treatment with Edmans reagent releases PTH-Gly.Carboxypeptidase A releases Phe.Treatment with cyanogen bromide yields the following three peptides:1. Gly-Leu-Tyr-Phe-Lys-Ser-Met 2. Gly-Leu-Tyr-Lys-Val-Ile-Arg-Met 3. Leu-Pro-PheTreatment with trypsin yields the following four peptides:1. Gly-Leu-Tyr-Phe-Lys 3. Val-Ile-Arg2. Ser-Met-Gly-Leu-Tyr-Lys 4. Met-Leu-Pro-Phe
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