A sample of adenosine triphosphate (ATP) (MW 507, e 5 14,700 M21cm21 at 257 nm)
Chapter 20, Problem 20.26(choose chapter or problem)
A sample of adenosine triphosphate (ATP) (MW 507, e 5 14,700 M21cm21 at 257 nm) isdissolved in 5.0 mL of buffer. A 250-mL aliquot is removed and placed in a 1 cm cuvettewith sufficient buffer to give a total volume of 2.0 mL. The absorbance of the sample at257 nm is 1.15. Calculate the weight of ATP in the original 5.0 mL sample.
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